Immediate and persistent transcriptional correlates of long-term sensitization training at different CNS loci in Aplysia californica.
Immediate and persistent transcriptional correlates of long-term sensitization training at different CNS loci in Aplysia californica.
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DOI：
10.1371/journal.pone.0114481
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Calin-Jageman IE
中科院分区：
文献类型：
--
作者：
Herdegen S;Conte C;Kamal S;Calin-Jageman RJ;Calin-Jageman IE
重复的伤害性刺激产生长期敏感的防御性退缩反射在Aaplasia californica，一种形式的长期记忆，需要在转录和翻译的变化。以前的工作已经确定了10个转录本，这些转录本在胸膜神经节中长期敏化训练后迅速上调。在这里，我们使用定量PCR开始检查这些转录变化是如何在不同的中枢神经系统基因座表达相关的防御性退缩反射在1和24小时后，长期的敏化训练。具体而言，我们从a）胸膜神经节的感觉楔中取样，该感觉楔仅包含VC伤害感受器细胞体，其有助于介导防御性撤回回路的输入，B）剩余的胸膜神经节，其包含撤回中间神经元，以及c）足神经节，其包含许多运动神经元。VC聚类的结果显示了不同的调节时间模式：1）C/EBP、C/EBPγ和CREB 1的海兔同源物的快速但短暂的上调，2）BiP、Tolloid/BMP-1和传感蛋白的延迟但持续的上调，3）快速和持续的上调Egr、GlyT 2、VPS 36和一种未表征的蛋白质（L0 C101862095），和4）钙调蛋白（CaM）和还原酶相关蛋白（RRP）的失活症同源物的出乎意料的缺乏调节。其余胸膜神经节的变化反映了1小时时VC簇中发现的变化，但调节水平减弱。由于这些样本几乎没有表达VC特异性转录传感蛋白，我们的数据表明，敏化训练可能会诱导防御性退缩中间神经元或与防御性退缩无关的神经元的转录变化。在足神经节，我们只观察到一个快速，但短暂的EGR表达增加，表明长期的敏化训练可能会诱导运动神经元的转录变化，但在这种类型的细胞不同的转录终点的可能性提高。
Repeated noxious stimulation produces long-term sensitization of defensive withdrawal reflexes in Aplysia californica, a form of long-term memory that requires changes in both transcription and translation. Previous work has identified 10 transcripts which are rapidly up-regulated after long-term sensitization training in the pleural ganglia. Here we use quantitative PCR to begin examining how these transcriptional changes are expressed in different CNS loci related to defensive withdrawal reflexes at 1 and 24 hours after long-term sensitization training. Specifically, we sample from a) the sensory wedge of the pleural ganglia, which exclusively contains the VC nociceptor cell bodies that help mediate input to defensive withdrawal circuits, b) the remaining pleural ganglia, which contain withdrawal interneurons, and c) the pedal ganglia, which contain many motor neurons. Results from the VC cluster show different temporal patterns of regulation: 1) rapid but transient up-regulation of Aplysia homologs of C/EBP, C/EBPγ, and CREB1, 2) delayed but sustained up-regulation of BiP, Tolloid/BMP-1, and sensorin, 3) rapid and sustained up-regulation of Egr, GlyT2, VPS36, and an uncharacterized protein (LOC101862095), and 4) an unexpected lack of regulation of Aplysia homologs of calmodulin (CaM) and reductase-related protein (RRP). Changes in the remaining pleural ganglia mirror those found in the VC cluster at 1 hour but with an attenuated level of regulation. Because these samples had almost no expression of the VC-specific transcript sensorin, our data suggests that sensitization training likely induces transcriptional changes in either defensive withdrawal interneurons or neurons unrelated to defensive withdrawal. In the pedal ganglia, we observed only a rapid but transient increase in Egr expression, indicating that long-term sensitization training is likely to induce transcriptional changes in motor neurons but raising the possibility of different transcriptional endpoints in this cell type.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1016/s0197-2456(98)00037-3
发表时间：
1998-12-01
期刊：
CONTROLLED CLINICAL TRIALS
影响因子：
--
作者：
Dupont, WD;Plummer, WD
通讯作者：
Plummer, WD
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
9.2
作者：
Abrams, Thomas W.
通讯作者：
Abrams, Thomas W.
影响因子：
3
作者：
Esdin, Joseph;Pearce, Kaycey;Glanzman, David L.
通讯作者：
Glanzman, David L.