Evidence for the antisense transcription in the proviral R29-127 strain of bovine immunodeficiency virus

Evidence for the antisense transcription in the proviral R29-127 strain of bovine immunodeficiency virus
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牛免疫缺陷病毒原病毒 R29-127 株中反义转录的证据

DOI:
10.1007/s12250-015-3559-6
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发表时间:
2015-04
期刊:
影响因子:
5.5
通讯作者:
Xiaohong Kong
Xiaohong Kong
中科院分区:
医学2区
文献类型:
--
作者:
Bin Liu;Xuechao Zhao;Wenyuan Shen;Xiaohong Kong

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总之,本报告记录了BIV R29株克隆127的反义转录物的产生。预测了一个反义ORF,并在用含有BIV 127前病毒序列的DNA转染的293 T细胞中检测到反义转录物。更重要的是,在BIV 127感染的BIVE细胞中检测到反义转录物。应该注意的是,本研究试图证明R29株BIV-127中存在反义转录物;因此,结果并不涉及其他BIV毒株中是否存在反义转录物。
In summary, this report documents the generation of antisense transcripts from clone127 of the R29 strain of BIV. An antisense ORF was predicted, and antisense transcripts were detected in 293T cells transfected with DNA containing the BIV127 proviral sequence. More importantly, the antisense transcript was detected in BIVE cells infected with BIV127. It should be noted that this research attempted to demonstrate the presence of antisense transcripts in BIV-127 of the R29 strain; thus, the results do not address whether the antisense transcripts exist in other BIV strains.
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