Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA

Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA
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基于微卫星DNA的中国对虾7个地理种群的遗传分化

DOI:
10.1016/j.aquaculture.2008.10.030
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发表时间:
2009-02
期刊:
影响因子:
4.5
通讯作者:
Liu Ping
Liu Ping
中科院分区:
农林科学1区
文献类型:
--
作者:
Jang In Kwon;Meng Xian Hong;Wang Qing Yin;Kong Jie;Liu Ping

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利用微卫星DNA标记对中国对虾的遗传多样性进行了研究。在黄渤海的辽东湾、渤海湾、海州湾、乳山湾、海洋岛渔场、朝鲜半岛半岛西岸和南岸7个海区共采集210尾虾类。在KS中鉴定出一个新的中国对虾种群。从7个基因座中共鉴定出109个等位基因。在49个群体位点中,有34个位点偏离了Hardy-Weinberg平衡。UPGMA聚类分析表明,BH与LD、RS与HZ的中国对虾亲缘关系较近,而KW与KS的中国对虾亲缘关系较远。遗传距离和AMOVA分析确定了三个地区的不同群体:渤海和黄海沿着中国海岸,韩国西海岸,韩国南海岸。这些结果与标记重捕数据和一些分子标记研究结果一致,但与线粒体基因方法的结果不同。
Microsatellite DNA markers were used to investigate the genetic diversity of Penaeus (Fenneropenaeus) chinensis, a topic of controversy in previous studies. A total of 210 individual shrimps were collected in seven geographic locations in the Yellow and Bohai Seas: Liaodong Bay (LD), Bohai Bay (BH), Haizhou Bay (HZ), Rushan Bay (RS), Haiyangdao fishing ground (HYD), the west coast (KW), and the south coast of the Korean Peninsula (KS). A new population of P. chinensis was identified in KS. A total of 109 alleles were identified from seven loci. Among the 49 population loci, 34 showed significant deviation from the Hardy–Weinberg equilibrium. Cluster analysis using UPGMA revealed a close genetic relationship of P. chinensis between BH and LD and between RS and HZ, while the shrimp from KW and KS diverged. Genetic distance and AMOVA analysis identified three areas with distinct populations: the Bohai and Yellow Seas along the Chinese coast; the west coast of Korea, and the south coast of Korea. These results are consistent with tagging–recapture data and some molecular DNA marker studies, but different from results by mitochondrial gene methods.
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