Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice.
Effects of splenectomy on spontaneously chronic pancreatitis in aly/aly mice.
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DOI：
10.1155/2010/614890
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发表时间：
2010
影响因子：
--
通讯作者：
Itoh M
中科院分区：
文献类型：
--
作者：
Wang HX;Yi SQ;Li J;Terayama H;Naito M;Hirai S;Qu N;Yi N;Itoh M
背景和目标。Aly/Aly突变小鼠的特点是缺乏淋巴结，Peyer's斑，和明确的淋巴滤泡在脾脏中被发现，在这项研究中，我们使用脾切除的Aly/Aly小鼠阐明的影响，次级淋巴器官的发展Aly/Aly自身免疫性胰腺炎。方法.将48只10周龄aly/aly小鼠分为脾切除组和假手术组。分别于术后20、30、40周龄对胰腺进行组织学和免疫组化分析。 结果结果表明，脾切除aly/aly小鼠20周龄时，单个核细胞浸润仅限于小叶间结缔组织，30、40周龄时浸润不明显。此外，与假aly/aly小鼠相比，在胰腺组织中检测到CD 4 + T、CD 8 + T和B细胞的表达明显降低，然而，在脾切除和未切除的小鼠之间，巨噬细胞的表达没有显著差异。 结论.脾切除后aly/aly小鼠胰腺炎的炎症浸润和发展受到有效抑制，这至少部分与抑制胰腺组织中T和B细胞的浸润有关，而与巨噬细胞无关。
Background and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer's patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the development of aly/aly autoimmune pancreatitis. Methods. Forty-eight 10-week-old aly/aly mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively. Results. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously at the age of 30 and 40 weeks in splenectomized aly/aly mice. Furthermore, an apparent decrease in the expressions of CD4+ T, CD8+ T, and B cells was detected in the pancreatic tissues compared with sham aly/aly mice, however, no significant difference in macrophage expression between mice with and without a splenectomy. Conclusions. Inflammation infiltration and development of the pancreatitis in aly/aly mice were suppressed effectively after splenectomy, which was, at least partly, correlated to inhibition of the infiltration of T and B cells in pancreatic tissues but not to macrophages.
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影响因子：
4.4
作者：
Adachi, S;Yoshida, H;Nishikawa, S
通讯作者：
Nishikawa, S
影响因子：
4.4
作者：
Sanchez-Valdepenas, Carmen;Martin, Angel G.;Fresno, Manuel
通讯作者：
Fresno, Manuel
DOI：
10.1073/pnas.0604183103
发表时间：
2006-09-05
影响因子：
11.1
作者：
Coles, Mark C.;Veiga-Fernandes, Henrique;Kioussis, Dimitris
通讯作者：
Kioussis, Dimitris
DOI：
10.1073/pnas.092596999
发表时间：
2002-04-30
影响因子：
11.1
作者：
Chalasani, G;Dai, ZH;Lakkis, FG
通讯作者：
Lakkis, FG
影响因子：
4.6
作者：
Kuranaga, N;Kinoshita, M;Seki, S
通讯作者：
Seki, S