Aquatic Pseudomonads Inhibit Oomycete Plant Pathogens of Glycine max.
Aquatic Pseudomonads Inhibit Oomycete Plant Pathogens of Glycine max.
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DOI：
10.3389/fmicb.2018.01007
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wildschutte H
中科院分区：
文献类型：
--
作者：
Wagner A;Norris S;Chatterjee P;Morris PF;Wildschutte H
大豆幼苗根腐病是由寄主特异性病原菌大豆疫霉（Phytophthora sojae）和大量的霉属（Pythium）引起的，是一种影响美国中西部大豆产量的重要经济病害。作物病原体生物防治策略的研究主要集中在土壤微生物产生的化合物上，然而，最近的研究表明，水生细菌表达的独特化合物可以有效地抑制多种病原体。基于这些观察，我们假设淡水假单胞菌菌株可能产生抑制卵菌生长的新型拮抗化合物。为了验证这一预测，我们从土壤和淡水生境中分离了330株假单胞菌，并测定了它们对5种卵菌的活性：大豆疫霉（Phytophthora sojae）、异thalicum、不规则皮霉（Pythium heterothalicum）、sylvaticum皮霉（Pythium sylvaticum）和最后皮霉（Pythium ultimum），这5种卵菌都对大豆具有致病性。其中118株对至少一种卵菌具有拮抗活性，16株对所有病原菌均有抑制作用。水生分离菌对卵菌的拮抗活性明显高于土壤分离菌。一种水源菌株06c126被预测表达铁载体，并在富含营养和缺铁的培养基上表现出不同的拮抗特征，这表明产生了不止一种有效抑制卵菌的化合物。这些结果支持了水生菌株是抑制病原体的化合物的有效来源的概念。我们概述了一种策略，以确定其他菌株表达独特的化合物，可能是有用的生物防治剂。
Seedling root rot of soybeans caused by the host-specific pathogen Phytophthora sojae, and a large number of Pythium species, is an economically important disease across the Midwest United States that negatively impacts soybean yields. Research on biocontrol strategies for crop pathogens has focused on compounds produced by microbes from soil, however, recent studies suggest that aquatic bacteria express distinct compounds that efficiently inhibit a wide range of pathogens. Based on these observations, we hypothesized that freshwater strains of pseudomonads might be producing novel antagonistic compounds that inhibit the growth of oomycetes. To test this prediction, we utilized a collection of 330 Pseudomonas strains isolated from soil and freshwater habitats, and determined their activity against a panel of five oomycetes: Phytophthora sojae, Pythium heterothalicum, Pythium irregulare, Pythium sylvaticum, and Pythium ultimum, all of which are pathogenic on soybeans. Among the bacterial strains, 118 exhibited antagonistic activity against at least one oomycete species, and 16 strains were inhibitory to all pathogens. Antagonistic activity toward oomycetes was significantly more common for aquatic isolates than for soil isolates. One water-derived strain, 06C 126, was predicted to express a siderophore and exhibited diverse antagonistic profiles when tested on nutrient rich and iron depleted media suggesting that more than one compound was produced that effectively inhibited oomycetes. These results support the concept that aquatic strains are an efficient source of compounds that inhibit pathogens. We outline a strategy to identify other strains that express unique compounds that may be useful biocontrol agents.
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影响因子：
6.7
作者：
Baltrus DA;Nishimura MT;Romanchuk A;Chang JH;Mukhtar MS;Cherkis K;Roach J;Grant SR;Jones CD;Dangl JL
通讯作者：
Dangl JL
影响因子：
5.2
作者：
Guyer A;De Vrieze M;Bönisch D;Gloor R;Musa T;Bodenhausen N;Bailly A;Weisskopf L
通讯作者：
Weisskopf L
影响因子：
2.2
作者：
Edgar, R. E.;Morris, P. F.;McKay, R. M. L.
通讯作者：
McKay, R. M. L.
影响因子：
4.4
作者：
Chatterjee, Payel;Davis, Elizabeth;Wildschutte, Hans
通讯作者：
Wildschutte, Hans
细菌素对尿道导管相关的假单胞菌生物膜的拮抗作用。
DOI：
10.4297/najms.2011.3367
发表时间：
2011-08
期刊：
North American journal of medical sciences
影响因子：
--
作者：
Al-Mathkhury HJ;Ali AS;Ghafil JA
通讯作者：
Ghafil JA