Exploring the relationship between autophagy and Gefitinib resistance in NSCLC by silencing PDLIM5 using ultrasound-targeted microbubble destruction technology.
Exploring the relationship between autophagy and Gefitinib resistance in NSCLC by silencing PDLIM5 using ultrasound-targeted microbubble destruction technology.
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利用超声靶向微泡破坏技术沉默 PDLIM5 探索 NSCLC 中自噬与吉非替尼耐药之间的关系
DOI：
10.1186/s12935-022-02718-4
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发表时间：
2022-09-25
影响因子：
5.8
通讯作者：
中科院分区：
文献类型：
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作者：
Background
Ultrasound-targeted microbubble destruction (UTMD) technology is a new drug and gene delivery strategy. This study investigates novel ultrasound (US) sensitive siRNA-loaded nanobubbles (siRNA-NBs) to explore the relationship between PDLIM5 mediated autophagy and drug resistance development using epidermal growth factor tyrosine kinase inhibitors (EGFR-TKIs) in the treatment of non-small cell lung cancer (NSCLC).
Methods
US sensitive siRNA-NBs were designed to inhibit the expression of PDLIM5 in gefitinib-resistant human NSCLC PC9GR cells in vitro. The expression of autophagy-related proteins (P62 and LC3-II/I) and autophagosomes in PC9GR cells after PDLIM5 gene silencing were explored.
Results
US-sensitive PDLIM5-targeted siRNA-NBs were effectively delivered into PC9GR cells, inhibiting PDLIM5 expression, increasing LC3-II/I and p62 expressions and increasing autophagosomes in PC9GR cells in vitro.
Conclusions
Using UTMD, US-sensitive siRNA-NBs have the potential as an ideal delivery vector to mediate highly effective RNA interference for NSCLC cells. Furthermore, PDLIM5 plays a role in the autophagy-mediated resistance in gefitinib-resistant PC9GR cells.
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DOI：
10.1158/1078-0432.ccr-18-2204
发表时间：
2019-04-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Haas NB;Appleman LJ;Stein M;Redlinger M;Wilks M;Xu X;Onorati A;Kalavacharla A;Kim T;Zhen CJ;Kadri S;Segal JP;Gimotty PA;Davis LE;Amaravadi RK
通讯作者：
Amaravadi RK
影响因子：
12.4
作者：
Sirsi SR;Borden MA
通讯作者：
Borden MA
影响因子：
5.8
作者：
Dubois, Charlotte;Kondratskyi, Artem;Vanden Abeele, Fabien
通讯作者：
Vanden Abeele, Fabien
影响因子：
5.2
作者：
Kang JI;Kim DH;Sung KW;Shim SM;Cha-Molstad H;Soung NK;Lee KH;Hwang J;Lee HG;Kwon YT;Kim BY
通讯作者：
Kim BY
影响因子：
6
作者：
Li Y;Du M;Fang J;Zhou J;Chen Z
通讯作者：
Chen Z