SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.
SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.
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DOI：
10.1016/j.devcel.2015.01.014
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发表时间：
2015-03-09
影响因子：
11.8
通讯作者：
Namekawa, Satoshi H.
中科院分区：
文献类型：
--
作者：
Hasegawa, Kazuteru;Sin, Ho-Su;Maezawa, So;Broering, Tyler J.;Kartashov, Andrey V.;Alavattam, Kris G.;Ichijima, Yosuke;Zhang, Fan;Bacon, W. Clark;Greis, Kenneth D.;Andreassen, Paul R.;Barski, Artem;Namekawa, Satoshi H.
配子发生依赖于种系特异性基因的表达。然而，目前尚不清楚种系表观基因组是如何从体细胞谱系中明显地建立起来的。在这里，我们表明，通常在体细胞谱系和精子发生祖细胞中表达的基因在男性生殖系的晚期以全基因组的方式受到抑制，并确定了潜在的机制。SCML2是Polycomb抑制复合体1 （PRC1）的生殖系特异性亚基，通过两种截然相反的机制建立了雄性生殖系独特的表观基因组。SCML2与PRC1一起作用，促进依赖rnf2的H2A泛素化，从而在常染色体上标记体细胞/祖基因进行抑制。矛盾的是，SCML2还可以在减数分裂期间阻止性染色体上依赖rnf2的H2A泛素化，从而使性染色体的独特表观遗传编程能够用于男性生殖。我们的研究结果揭示了不同的机制，包括一个共同的调节，通过这个调节，男性生殖系表观基因组与体细胞和祖细胞的表观基因组区分开来。
Gametogenesis is dependent on the expression of germline-specific genes. However, it remains unknown how the germline epigenome is distinctly established from that of somatic lineages. Here we show that genes commonly expressed in somatic lineages and spermatogenesis-progenitor cells undergo repression in a genome-wide manner in late stages of the male germline and identify underlying mechanisms. SCML2, a germline-specific subunit of a Polycomb repressive complex 1 (PRC1), establishes the unique epigenome of the male germline through two distinct antithetical mechanisms. SCML2 works with PRC1 and promotes RNF2-dependent ubiquitination of H2A, thereby marking somatic/progenitor genes on autosomes for repression. Paradoxically, SCML2 also prevents RNF2-dependent ubiquitination of H2A on sex chromosomes during meiosis, thereby enabling unique epigenetic programming of sex chromosomes for male reproduction. Our results reveal divergent mechanisms involving a shared regulator by which the male germline epigenome is distinguished from that of the soma and progenitor cells.
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影响因子：
11.8
作者：
Lu, Lin-Yu;Wu, Jiaxue;Ye, Lin;Gavrilina, Galina B.;Saunders, Thomas L.;Yu, Xiaochun
通讯作者：
Yu, Xiaochun
影响因子：
16
作者：
Lou, ZK;Minter-Dykhouse, K;Chen, JJ
通讯作者：
Chen, JJ
影响因子：
30.8
作者：
Khil, PP;Smirnova, NA;Camerini-Otero, RD
通讯作者：
Camerini-Otero, RD
影响因子：
7.7
作者：
Bonasio R;Lecona E;Narendra V;Voigt P;Parisi F;Kluger Y;Reinberg D
通讯作者：
Reinberg D
DOI：
10.3791/1293
发表时间：
2009-08-19
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Choo, Yeun Su;Zhang, Zhuohua
通讯作者：
Zhang, Zhuohua