Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ.
Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ.
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DOI：
10.1242/jcs.260249
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发表时间：
2022-07-15
影响因子：
4
通讯作者：
O'Brien, Lucy Erin
中科院分区：
文献类型：
--
作者：
Kim, Anna A.;Nguyen, Amanda;Marchetti, Marco;Du, XinXin;Montell, Denise J.;Pruitt, Beth L.;O'Brien, Lucy Erin
胞浆Ca 2+是一种高度动态、严格调控和广泛保守的细胞信号。Ca 2+动力学已经在细胞单培养中被广泛研究，然而体内器官包括干细胞和分化细胞的异质群体。在这里，我们研究Ca 2+动力学在成年果蝇肠道，自我更新的上皮器官，干细胞不断产生的女儿，分化成肠内分泌细胞或肠上皮细胞。离体的整个器官的实时成像显示，干细胞子细胞在分化后采用了截然不同的Ca 2+振荡模式：肠内分泌细胞表现出单细胞Ca 2+振荡，而肠细胞表现出有节奏的长距离Ca 2+波。这些多细胞波不通过未成熟的祖细胞（干细胞和肠母细胞）传播，其振荡频率约为肠内分泌细胞的一半。间隙连接的器官级抑制消除了所有细胞类型中的Ca 2+振荡-甚至，有趣的是，在仅被肠上皮细胞包围的祖细胞和肠内分泌细胞中。我们的研究结果表明，细胞采用命运特异性模式的Ca 2+动力学，因为他们最终分化，并揭示了不同类型的细胞在一个单一的，连贯的上皮细胞的振荡动力学是独立起搏。总结：果蝇肠上皮细胞的实时成像显示，干细胞及其终端后代表现出独特的，命运特异性的Ca 2+振荡，节奏彼此独立。
Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. Ca2+ dynamics have been studied widely in cellular monocultures, yet organs in vivo comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging of whole organs ex vivo reveals that stem-cell daughters adopt strikingly distinct patterns of Ca2+ oscillations after differentiation: enteroendocrine cells exhibit single-cell Ca2+ oscillations, whereas enterocytes exhibit rhythmic, long-range Ca2+ waves. These multicellular waves do not propagate through immature progenitors (stem cells and enteroblasts), of which the oscillation frequency is approximately half that of enteroendocrine cells. Organ-scale inhibition of gap junctions eliminates Ca2+ oscillations in all cell types – even, intriguingly, in progenitor and enteroendocrine cells that are surrounded only by enterocytes. Our findings establish that cells adopt fate-specific modes of Ca2+ dynamics as they terminally differentiate and reveal that the oscillatory dynamics of different cell types in a single, coherent epithelium are paced independently. Summary: Live imaging of the Drosophila intestinal epithelium reveals that stem cells and their terminal progeny exhibit distinct, fate-specific Ca2+ oscillations that are paced independently of each other.
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影响因子：
16.6
作者：
Crameri F;Shephard GE;Heron PJ
通讯作者：
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作者：
Dutta, Devanjali;Dobson, Adam J.;Buchon, Nicolas
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48
作者：
de Chaumont, Fabrice;Dallongeville, Stephane;Olivo-Marin, Jean-Christophe
通讯作者：
Olivo-Marin, Jean-Christophe
影响因子：
7.7
作者：
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通讯作者：
Kim, Douglas S.
影响因子：
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作者：
HAROOTUNIAN, AT;KAO, JPY;TSIEN, RY
通讯作者：
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