Alphacoronaviruses in New World bats: prevalence, persistence, phylogeny, and potential for interaction with humans.
Alphacoronaviruses in New World bats: prevalence, persistence, phylogeny, and potential for interaction with humans.
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DOI：
10.1371/journal.pone.0019156
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发表时间：
2011-05-12
期刊：
影响因子：
3.7
通讯作者：
Dominguez SR
中科院分区：
文献类型：
--
作者：
Osborne C;Cryan PM;O'Shea TJ;Oko LM;Ndaluka C;Calisher CH;Berglund AD;Klavetter ML;Bowen RA;Holmes KV;Dominguez SR
蝙蝠是许多不同的冠状病毒（CoV）以及许多其他重要的人畜共患病毒的宿主。从2007年到2009年，我们对来自科罗拉多21个不同地点的2科17种1,044只食虫蝙蝠的粪便和/或肛拭子进行了采样。我们在4种蝙蝠中检测到了冠状病毒RNA：大棕蝠（Eptesicus fuscus），10%的患病率;长腿蝙蝠（鼠耳蝠），8%的患病率;小棕蝠（鼠耳蝠lucifugus），3%的患病率;和西方长耳蝙蝠（鼠耳蝠evotis），2%的患病率。总体而言，幼年蝙蝠对CoV RNA呈阳性的可能性是成年蝙蝠的两倍。在两个农村采样点，在本研究的三个连续的夏天，在大棕色和长腿蝙蝠中检测到CoV RNA。在每个测试期内，在所有五个城市产妇栖息地的大棕色蝙蝠中检测到CoV RNA。单独标记的大棕色蝙蝠对CoV RNA呈阳性，后来再次取样，所有这些都变成了CoV RNA阴性。RdRp基因的核苷酸序列分为3个主要簇，都与旧大陆蝙蝠不同。在大棕蝙蝠和长腿蝙蝠的基因1b和刺突基因的扩增子中发现了相似的核苷酸序列，这表明CoV可能能够感染不同属的蝙蝠。这些数据表明，蝙蝠中冠状病毒的持续进化创造了持续威胁其他物种宿主的可能性。在靠近人类居住地的栖息地（10%）和已知与人类直接接触（19%）的大棕蝙蝠中检测到高流行率的α冠状病毒RNA，这表明这些病毒存在跨物种传播的重大潜在机会。在整个美洲的蝙蝠中进行进一步的CoV监测研究是必要的。
Bats are reservoirs for many different coronaviruses (CoVs) as well as many other important zoonotic viruses. We sampled feces and/or anal swabs of 1,044 insectivorous bats of 2 families and 17 species from 21 different locations within Colorado from 2007 to 2009. We detected alphacoronavirus RNA in bats of 4 species: big brown bats (Eptesicus fuscus), 10% prevalence; long-legged bats (Myotis volans), 8% prevalence; little brown bats (Myotis lucifugus), 3% prevalence; and western long-eared bats (Myotis evotis), 2% prevalence. Overall, juvenile bats were twice as likely to be positive for CoV RNA as adult bats. At two of the rural sampling sites, CoV RNAs were detected in big brown and long-legged bats during the three sequential summers of this study. CoV RNA was detected in big brown bats in all five of the urban maternity roosts sampled throughout each of the periods tested. Individually tagged big brown bats that were positive for CoV RNA and later sampled again all became CoV RNA negative. Nucleotide sequences in the RdRp gene fell into 3 main clusters, all distinct from those of Old World bats. Similar nucleotide sequences were found in amplicons from gene 1b and the spike gene in both a big-brown and a long-legged bat, indicating that a CoV may be capable of infecting bats of different genera. These data suggest that ongoing evolution of CoVs in bats creates the possibility of a continued threat for emergence into hosts of other species. Alphacoronavirus RNA was detected at a high prevalence in big brown bats in roosts in close proximity to human habitations (10%) and known to have direct contact with people (19%), suggesting that significant potential opportunities exist for cross-species transmission of these viruses. Further CoV surveillance studies in bats throughout the Americas are warranted.
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影响因子：
11.8
作者：
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