Single and Double Mutations in Tomato Ripening Transcription Factors Have Distinct Effects on Fruit Development and Quality Traits.
Single and Double Mutations in Tomato Ripening Transcription Factors Have Distinct Effects on Fruit Development and Quality Traits.
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DOI：
10.3389/fpls.2021.647035
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发表时间：
2021
影响因子：
5.6
通讯作者：
Blanco-Ulate B
中科院分区：
文献类型：
--
作者：
Adaskaveg JA;Silva CJ;Huang P;Blanco-Ulate B
与番茄转录因子无色不成熟(SPL-CNR)、不成熟(NAC-NOR)和成熟抑制因子(MADS-RIN)相关的自发突变导致果实不经历正常的成熟特征，但在表型上是可区分的。在这里，我们扩展了成熟突变对果实成熟后发育的生理、分子和遗传影响的知识。我们通过表型和转录组分析表明，Cnr果实在果实成熟之前表现出广泛的发育缺陷，但果实仍经历一些类似于野生型的成熟变化。因此，Cnr应该被认为是一个果实发育突变，而不仅仅是一个成熟的突变。此外，我们还发现，在NOR和RIN突变的果实中，一些成熟过程发生在衰老过程中，这表明虽然这些突变的一些成熟过程受到抑制，但另一些只是被推迟了。通过基因表达分析和激素的直接测定，我们发现Cnr、NOR和Rin在植物激素的代谢和信号转导方面都有变化。CNR突变体产生比基础水平更多的乙烯，而NOR和RIN积累高浓度的脱落酸。为了确定突变之间的遗传相互作用，我们首次创建了纯合子双突变体。双成熟突变体的表型分析表明，Cnr对果实性状有很强的影响，NOR和Rin的结合导致了一个中等成熟的突变体表型。然而，我们发现突变之间的遗传相互作用比预期的要复杂得多，因为Cnr/Nor双突变果实具有Cnr表型，但与Nor果一样，对成熟相关基因的表达具有抑制作用。我们对Cnr、Nor和Rin突变体的重新评估为利用这些突变体研究果实发育及其在番茄果实品质育种中的意义提供了新的见解。
Spontaneous mutations associated with the tomato transcription factors COLORLESS NON-RIPENING (SPL-CNR), NON-RIPENING (NAC-NOR), and RIPENING-INHIBITOR (MADS-RIN) result in fruit that do not undergo the normal hallmarks of ripening but are phenotypically distinguishable. Here, we expanded knowledge of the physiological, molecular, and genetic impacts of the ripening mutations on fruit development beyond ripening. We demonstrated through phenotypic and transcriptome analyses that Cnr fruit exhibit a broad range of developmental defects before the onset of fruit ripening, but fruit still undergo some ripening changes similar to wild type. Thus, Cnr should be considered as a fruit developmental mutant and not just a ripening mutant. Additionally, we showed that some ripening processes occur during senescence in the nor and rin mutant fruit, indicating that while some ripening processes are inhibited in these mutants, others are merely delayed. Through gene expression analysis and direct measurement of hormones, we found that Cnr, nor, and rin have alterations in the metabolism and signaling of plant hormones. Cnr mutants produce more than basal levels of ethylene, while nor and rin accumulate high concentrations of abscisic acid. To determine genetic interactions between the mutations, we created for the first time homozygous double mutants. Phenotypic analyses of the double ripening mutants revealed that Cnr has a strong influence on fruit traits and that combining nor and rin leads to an intermediate ripening mutant phenotype. However, we found that the genetic interactions between the mutations are more complex than anticipated, as the Cnr/nor double mutant fruit has a Cnr phenotype but displayed inhibition of ripening-related gene expression just like nor fruit. Our reevaluation of the Cnr, nor, and rin mutants provides new insights into the utilization of the mutants for studying fruit development and their implications in breeding for tomato fruit quality.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
比较 WGBS 鉴定了影响番茄表突变体中不成熟表型的基因 无色不成熟
影响因子：
9.1
作者：
Chen, Weiwei;Yu, Zhiming;Hong, Yiguo
通讯作者：
Hong, Yiguo
影响因子：
5.8
作者：
Le, Sebastien;Josse, Julie;Husson, Francois
通讯作者：
Husson, Francois
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
11.6
作者：
Fujisawa, Masaki;Nakano, Toshitsugu;Ito, Yasuhiro
通讯作者：
Ito, Yasuhiro