Effects of a manganese (III) porphyrin catalytic antioxidant in a mouse closed head injury model.
Effects of a manganese (III) porphyrin catalytic antioxidant in a mouse closed head injury model.
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锰 (III) 卟啉催化抗氧化剂在小鼠闭合性头部损伤模型中的作用。
DOI：
10.1016/j.ejphar.2005.12.031
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Warner,DavidS
中科院分区：
文献类型：
--
作者：
Leinenweber,StephenB;Sheng,Huaxin;Lynch,JohnR;Wang,Haichen;Batinic-Haberle,Ines;Laskowitz,DanielT;Crapo,JamesD;Pearlstein,RobertD;Warner,DavidS
闭合性颅脑损伤可导致脑组织氧化应激。在小鼠闭合性脑损伤模型中，评价了一种锰(III)卟啉催化抗氧化剂的效果。小鼠造成闭合性颅脑损伤，15min后静注。赋形剂或3 mg/kg MnTe-2-PyP5+。测定乌头酸酶活性、荧光翡翠染色、胶质纤维酸性蛋白免疫反应性和转杆下降潜伏期。闭合性颅脑损伤改变了所有变量。除闭合性颅脑损伤后2小时乌头酸酶失活减弱外，MnTe-2-PyP5+对其他指标均无影响。在第二个实验中，小鼠接受3 mg/kg或6 mg/kg的MnTE-2-PyP5+或赋形剂静脉注射。闭合性颅脑损伤后15min。测定旋转迷宫和Morris水迷宫的潜伏期。闭合性头部损伤改变了两项测试的表现。未观察到MnTe-2-PyP5+有统计学意义的影响。我们的结论是，单次给予MnTE-2-PyP5+不会改变小鼠闭合性脑损伤模型的结局。
Closed head injury induces cerebral oxidative stress. The efficacy of a Mn (III) porphyrin catalytic antioxidant was assessed in a mouse closed head injury model. Mice were subjected to closed head injury and treated 15 min later with an i.v. bolus of vehicle or 3 mg/kg MnTE-2-PyP5+. Aconitase activity, Fluoro-Jade staining, glial fibrillary acidic protein immunoreactivity, and rotarod falling latencies were measured. Closed head injury altered all variables. MnTE-2-PyP5+had no effect on any variable with the exception of attenuation of aconitase inactivation at 2 h post-closed head injury. In a second experiment, mice received 3 mg/kg or 6 mg/kg MnTE-2-PyP5+or vehicle i.v. 15 min post-closed head injury. Rotarod and Morris water maze latencies were measured. Closed head injury altered performance in both tests. No statistically significant effect of MnTE-2-PyP5+was observed. We conclude that single dose MnTE-2-PyP5+does not alter outcome in this mouse closed head injury model.
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影响因子：
--
作者：
H. Sheng;Ines Batinc;D. Warner
通讯作者：
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