Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy.
Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy.
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鞘氨醇激酶 1 的过度表达与唾液腺癌进展相关，可能是辅助治疗的新型预测标志物
DOI：
10.1186/1471-2407-10-495
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发表时间：
2010-09-16
期刊：
影响因子：
3.8
通讯作者：
Song L
中科院分区：
文献类型：
--
作者：
Liu G;Zheng H;Zhang Z;Wu Z;Xiong H;Li J;Song L
鞘氨醇激酶-1 （SPHK1）的过度表达已被证明与多种类型人类癌症的发生和进展有关。本研究旨在表征SPHK1在唾液腺癌（SGC）中的表达，并探讨SPHK1表达与SGC进展之间的关系。方法采用实时荧光定量PCR和western blot方法检测2例正常唾液腺组织、8例不同临床分期SGC组织和5对原发SGC及相邻唾液腺组织中SPHK1的表达。应用免疫组化方法分析了159例临床病理特征的SGC患者中SPHK1蛋白的表达。进行统计分析以确定预后和诊断相关性。结果sphk1在SGC组织中的mRNA和蛋白表达水平均明显高于正常唾液腺组织和配对邻近唾液腺组织。统计学分析显示SPHK1表达与临床分期（P= 0.005）、T分期（P= 0.017）、N分期（P= 0.009）、M分期（P= 0.002）、病理分型（P= 0.013）有显著相关性。SPHK1高表达的患者总生存时间较短，而SPHK1低表达的患者总生存时间较长。重要的是，未接受辅助治疗的SPHK1高表达组患者的总生存率明显低于SPHK1低表达组。此外，多因素分析提示SPHK1表达可能是SGC患者生存的独立预后指标。结论研究结果提示SPHK1表达与SGC进展相关，可作为SGC患者辅助治疗的一种新的、有价值的预测指标。
BackgroundOverexpression of sphingosine kinase-1 (SPHK1) has been demonstrated to be associated with the development and progression in various types of human cancers. The current study was to characterize the expression of SPHK1 in salivary gland carcinomas (SGC) and to investigate the association between SPHK1 expression and progression of SGC.MethodsThe expression of SPHK1 was examined in 2 normal salivary gland tissues, 8 SGC tissues of various clinical stages, and 5 pairs of primary SGC and adjacent salivary gland tissues from the same patient, using real-time PCR and western blot analysis. Furthermore, the SPHK1 protein expression was analyzed in 159 clinicopathologically characterized SGC cases by immunohistochemistry. Statistical analyses were performed to determine the prognostic and diagnostic associations.ResultsSPHK1 expression was found to be markedly upregulated in SGC tissues than that in the normal salivary gland tissues and paired adjacent salivary gland tissues, at both mRNA and protein levels. Statistical analysis revealed a significant correlation of SPHK1 expression with the clinical stage (P= 0.005), T classification (P= 0.017), N classification (P= 0.009), M classification (P= 0.002), and pathological differentiation (P= 0.013). Patients with higher SPHK1 expression had shorter overall survival time, whereas patients with lower SPHK1 expression had better survival. Importantly, patients in the group without adjuvant therapy who exhibited high SPHK1 expression had significantly lower overall survival rates compared with those with low SPHK1 expression. Moreover, multivariate analysis suggested that SPHK1 expression might be an independent prognostic indicator for the survival of SGC patients.ConclusionsOur results suggest that SPHK1 expression is associated with SGC progression, and might represent as a novel and valuable predictor for adjuvant therapy to SGC patients.
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鞘氨醇激酶表达会增加细胞内鞘氨醇1-磷酸盐，并促进细胞生长和存活。
DOI：
10.1083/jcb.147.3.545
发表时间：
1999-11-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Olivera A;Kohama T;Edsall L;Nava V;Cuvillier O;Poulton S;Spiegel S
通讯作者：
Spiegel S
影响因子：
56.9
作者：
Lee, MJ;Van Brocklyn, JR;Hla, T
通讯作者：
Hla, T
DOI：
10.1016/j.bbrc.2006.02.070
发表时间：
2006-04-21
影响因子：
3.1
作者：
Akao, Y;Banno, Y;Nozawa, Y
通讯作者：
Nozawa, Y
影响因子：
11.4
作者：
Bonhoure, E.;Lauret, A.;Cuvillier, O.
通讯作者：
Cuvillier, O.
DOI：
10.2310/7070.2003.11438
发表时间：
2003-10-01
期刊：
JOURNAL OF OTOLARYNGOLOGY
影响因子：
--
作者：
Nguyen, LHP;Black, MJ;Rochon, L
通讯作者：
Rochon, L