Morphological analysis of young and old pellicles of Salmonella Typhimurium
Morphological analysis of young and old pellicles of Salmonella Typhimurium
复制标题
鼠伤寒沙门氏菌新老菌膜形态分析
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Yaron
中科院分区：
文献类型：
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作者：
K. Scher;E. Kesselman;E. Shimoni;S. Yaron
各种各样的微生物能够在空气和液体之间的界面形成生物膜（膜）。本研究分析了鼠伤寒沙门菌胞膜成熟过程中的变化，并评价了纤维素在胞膜结构和形态中的作用。利用原子力显微镜和扫描电镜对膜的两侧形貌进行了表征。总体而言，面向水（WF）和面向空气（AF）的生物膜表面形态有显著差异。虽然AF侧看起来是均匀的，并且被细胞外涂层广泛覆盖，但WF侧的细胞分布成簇，覆盖较少。然而，细胞膜两侧的单细胞在大小和形状上的相似性可能表明细胞膜两侧的细菌细胞具有相似的生理状态。在成熟过程中，在膜上形成具有多个裂纹和通道的多孔结构，导致破裂。通过与纤维素缺乏突变体的细胞膜结构比较，证明了观察到的成熟细胞膜的解体可能部分是由基质成分的自水解引起的。
Abstract A wide variety of microorganisms are able to form biofilms at the interface between air and liquid (pellicles). In this study changes during the maturation of the pellicle of Salmonella Typhimurium were analysed and the role of cellulose in the pellicle structure and morphology evaluated. The morphology of both sides of the pellicle was characterised using atomic force microscopy and scanning electron microscopy. Overall, there was a marked difference in the morphology of the water-facing (WF) and air-facing (AF) biofilm surfaces. While the AF side appeared to be uniform, and extensively covered with an exocellular coating, cells in the WF side were distributed into clusters and were less covered. However, the similarity in size and shape of single cells from both sides of the pellicle may indicate that the bacterial cells across the pellicle have a similar physiological status. During maturation, porous structures with multiple cracks and channels were created in the pellicle, leading to disintegration. By comparison with the structure of pellicles of a cellulose-deficient mutant, it was demonstrated that the observed disintegration of mature pellicles probably occurred in part by self-hydrolysis of components of the matrix.
DOI：
10.1073/pnas.96.7.4028
发表时间：
1999-03-30
影响因子：
11.1
作者：
Yildiz, FH;Schoolnik, GK
通讯作者：
Schoolnik, GK