Occurrence and distribution of plankton-associated and free-living toxigenic Vibrio cholerae in a tropical estuary of a cholera endemic zone
Occurrence and distribution of plankton-associated and free-living toxigenic Vibrio cholerae in a tropical estuary of a cholera endemic zone
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霍乱流行区热带河口浮游生物相关和自由生活的产毒霍乱弧菌的出现和分布
DOI：
10.1007/s11273-012-9247-5
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发表时间：
2012
影响因子：
1.8
通讯作者：
Yamasaki
中科院分区：
文献类型：
--
作者：
Yamasaki
霍乱的流行被认为是受河口霍乱弧菌数量变化的影响。我们调查了孟加拉国卡纳富利河口在季风季节前后这种细菌的丰度和分布，这种细菌是“自由生活”（<20 μm部分）并与微型浮游植物（>20 μm）或浮游动物（>60 μm）相关。在高盐区（19-23实用盐度单位，PSU），可培养的弧菌种群约为102- 104菌落形成单位（CFU）ml− 1，在淡水中下降（<101 CFU ml−1）。非培养物依赖性检测产黄单胞病毒。霍乱O 1和O 139血清型的“自由生活”丰度（104- 105个细胞l−1）高于附着在浮游生物上的丰度（101- 103个细胞l−1）。然而，“自由生活”的O 1和O 139细胞有时缺席中盐和淡水地区（0.0-11实用盐度单位[PSU]）。相比之下，浮游生物样本总是窝藏这些血清型，尽管盐度和其他物理化学性质的变化。微型浮游植物和浮游动物分别以硅藻和蓝藻、桡足类和轮虫为主。毒性V.霍乱弧菌丰度与浮游生物丰度或种类无关，但与<20 μm的甲壳素含量呈正相关，其中悬浮颗粒物（SPM）、霍乱弧菌和甲壳素含量最高。C：N比值表明，SPM中的有机质主要来自浮游生物。“自由生活”和依附的差异发生。霍乱弧菌在霍乱中起着关键作用。霍乱蔓延到淡水地区。这种病原体与微型浮游生物（<20 μm）部分中的生物和颗粒的可能联系需要验证V的“自由生活”状态的概念。水生环境中的霍乱。
Cholera epidemics are thought to be influenced by changes in populations of estuarineVibrio cholerae. We investigated the abundance and distribution of this bacterium, as “free-living” (<20 μm fraction) and associated with microphytoplankton (>20 μm) or zooplankton (>60 μm), in the Karnaphuli estuary of Bangladesh during pre- and post-monsoon seasons. CultivableVibriopopulations were ~102–104colony forming units (CFU) ml−1in the high saline zone (19–23 practical salinity unit, PSU) and declined in freshwater (<101CFU ml−1). Culture independent detection of toxigenicV. choleraeO1 and O139 serogroups revealed a higher abundance of “free-living” (104–105cells l−1) than those attached to plankton (101–103cells l−1). However, “free-living” O1 and O139 cells were sometimes absent in the medium saline and freshwater areas (0.0–11 practical salinity unit [PSU]). In contrast, plankton samples always harbored these serogroups despite changes in salinity and other physico-chemical properties. Microphytoplankton and zooplankton were dominated by diatoms and blue-green algae, and copepods and rotifers, respectively. ToxigenicV. choleraeabundance did not correlate with plankton abundance or species but had a positive correlation with chitin in the <20 μm fraction, where suspended particulate matter (SPM),V. choleraeand chitin concentrations were highest. C:N ratios indicated that organic matter in SPM originated predominantly from plankton. The differential occurrence of “free-living” and attachedV. choleraesuggests a pivotal function of plankton inV. choleraespreading into freshwater areas. The probable association of this pathogen with organisms and particles in the nanoplankton (<20 μm) fraction requires validation of the concept of the “free living” state ofV. choleraein aquatic habitats.
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影响因子：
5.1
作者：
Gil, AI;Louis, VR;Colwell, RR
通讯作者：
Colwell, RR
影响因子：
56.9
作者：
COLWELL, RR;KAPER, J;JOSEPH, W
通讯作者：
JOSEPH, W
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
Y. Samiullah
通讯作者：
Y. Samiullah
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
M. S. Islam;M. Alam;S. Khan;A. Huq
通讯作者：
A. Huq
血凝非 El Tor 霍乱弧菌的分离
影响因子：
3.2
作者：
S. Rizvi;M. Huq;A. Benenson
通讯作者：
A. Benenson