Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues
Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues
复制标题
AKR 小鼠亚系中 AKR 小鼠白血病病毒序列的表征以及肿瘤组织中整合重组基因组的结构
作者：
Wim Quint;Wim Quax;H. V. D. Putten;Anton Berns
制备了AKR鼠白血病病毒（AKR-MLV）的特异性cDNA探针，用于检测AKR小鼠不同亚系正常组织和肿瘤组织中AKR-MLV序列。AKR菌株含有多达6个内源性AKR-MLV基因组。所有测试的亚株有一个共同的AKR-MLV基因座，密切相关的亚株有几个前病毒整合在一个相同的网站。AKR/FuRdA和AKR/JS株中病毒诱导的肿瘤显示出个体动物特有的AKR-MLV序列的重新整合模式，表明肿瘤的单克隆起源。对来自AKR/FuRdA和AKR/JS亚株的肿瘤DNA进行分析，在前病毒基因组内用限制性酶切割，揭示了正常组织中不存在的新的EcoRI限制性位点和BamHI限制性位点。这些位点的位置与整合的莫洛尼重组体中EcoRI和BamHI的切割位点以及分离的AKR貂细胞病灶形成病毒的结构相对应。所有的肿瘤分析数据包含几乎相同的整合重组基因组，这表明重组体的形成和白血病的过程之间的因果关系。
A specific cDNA probe of AKR murine leukemia virus (AKR-MLV) was prepared to detect AKR-MLV sequences in normal and tumor tissues in a variety of AKR mouse substrains. AKR strains contained up to six endogenous AKR-MLV genomes. All substrains tested had one AKR-MLV locus in common, and closely related substrains had several proviruses integrated in an identical site. Virus-induced tumors in the AKR/FuRdA and AKR/JS strains showed a reintegration pattern of AKR-MLV sequences unique for the individual animal, suggesting a monoclonal origin for the outgrown tumors. An analysis of tumor DNAs from the AKR/FuRdA and AKR/JS substrains with restriction enzymes cleaving within the proviral genome revealed a new EcoRI restriction site and BamHI restriction site not present in normal tissues. The positions of these sites corresponded both with cleavage sites of EcoRI and BamHI in integrated Moloney recombinants and with the structure of isolated AKR mink cell focus-forming viruses. All tumors analyzed to data contain nearly identical integrated recombinant genomes, suggesting a causal relationship between the formation of recombinants and the leukemogenic process.
水貂细胞焦点诱导鼠 C 型病毒的基因组分析：进展报告。
DOI：
10.1101/sqb.1980.044.01.138
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Lung,ML;Hering,C;Hartley,JW;Rowe,WP;Hopkins,N
通讯作者：
Hopkins,N