Changes in FGF-2 Expression in the Distal Spinal Cord Stump After Complete Cord Transection: A Comparison Between Infant and Adult Rats
Changes in FGF-2 Expression in the Distal Spinal Cord Stump After Complete Cord Transection: A Comparison Between Infant and Adult Rats
复制标题
脊髓完全横断后远端脊髓残端 FGF-2 表达的变化：婴儿和成年大鼠之间的比较
作者：
Mei;Y. Wakabayashi;H. Haro;K. Shinomiya
研究设计.应用逆转录聚合酶链反应（RT-PCR）和免疫组化方法，观察成纤维细胞生长因子-2（FGF-2）在幼年和成年大鼠脊髓远端横断损伤中的表达。Objective.探讨成纤维细胞生长因子2（FGF-2）在幼年和成年大鼠脊髓远端横断损伤中的表达规律。背景数据总结。幼年和成年大鼠脊髓下行纤维的再生能力不同。一种解释是，不同水平的FGF-2，一种促进神经突生长和修复的重要神经营养因子，在大鼠的远端横断脊髓中表达，为切断的轴突提供不同水平的支持。材料和方法。将幼年和成年大鼠的脊髓完全横断。在12、24、72小时和1周时，取出远端脊髓组织段，通过RT-PCR评估FGF-2 mRNA的表达。通过免疫染色确定FGF-2的分布和FGF-2阳性细胞的表型。结果FGF-2 mRNA的表达被证明是上调的远端脊髓的幼年大鼠，但不是成年大鼠。免疫组织化学分析显示，幼年大鼠脊髓远侧部神经元富含FGF-2免疫反应性（IR），而成年大鼠神经元中几乎未观察到FGF-2 IR，但在白色物质中观察到少数FGF-2阳性星形胶质细胞。结论脊髓全横断后，幼年大鼠远侧脊髓中FGF-2的表达高于成年大鼠。这可能为轴突延伸和功能恢复提供神经营养支持。
Study Design. Expression patterns of fibroblast growth factor-2 (FGF-2) in distal transected spinal cord in infant and adult rats were determined by reverse-transcription polymerase chain reaction (RT-PCR) and immunostaining. Objective. To reveal the expression pattern of FGF-2 in distal transected cord of infant and adult rats. Summary of Background Data. Descending fibers in the spinal cord of infant and adult rats show different regenerative capacity. One explanation is that different levels of FGF-2, an important neurotrophic factor for promoting neurite outgrowth and repair, are expressed in the distal transected cords of the rats, providing different levels of support for severed axons. Materials and Methods. Spinal cords of infant and adult rats were completely transected. At 12, 24, and 72 hours and at 1 week, segments of distal spinal cord tissues were removed and expression of FGF-2 mRNA was evaluated by RT-PCR. The distribution of FGF-2 and the phenotype of FGF-2-positive cells were determined by immunostaining. Results. Expression of FGF-2 mRNA was shown to be up-regulated in the distal cord of infant rats but not adult rats. Immunohistochemical analysis showed that neurons in distal cord of infant rats were rich in FGF-2 immunoreactivity (IR), whereas in adult rat neurons FGF-2 IR was hardly observed at all, although a few FGF-2-positive astrocytes were observed in the white matter. Conclusion. After complete spinal cord transection, the expression of FGF-2 in the distal cord of infant rats was high compared with that of adults. This may provide neurotrophic support for axonal extension and functional recovery.
影响因子：
1.7
作者：
J. J. Ramirez-J.;S. Finklestein;J. Keller;W. Abrams;M. George;T. Parakh
通讯作者：
J. J. Ramirez-J.;S. Finklestein;J. Keller;W. Abrams;M. George;T. Parakh
Interleukin-1 beta 增加成年大鼠大脑和器官型海马培养物中碱性成纤维细胞生长因子 mRNA 的表达。
DOI：
10.1016/0169-328x(94)90179-1
发表时间：
1994
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Rivera,S;Gold,SJ;Gall,CM
通讯作者：
Gall,CM
DOI：
10.1016/0169-328x(94)90026-4
发表时间：
1994-03-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
FOLLESA, P;WRATHALL, JR;MOCCHETTI, I
通讯作者：
MOCCHETTI, I