Molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, Aedes aegypti.
Molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, Aedes aegypti.
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DOI：
10.1038/s41598-018-20517-8
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发表时间：
2018-02-01
影响因子：
4.6
通讯作者：
Paluzzi JP
中科院分区：
文献类型：
--
作者：
Wahedi A;Paluzzi JP
最近发现的脂肪运动激素/考拉佐宁相关肽（ACP）是一种昆虫神经肽，结构介于考拉佐宁（CRZ）和脂肪运动（AKH）激素之间，它们都与脊椎动物促性腺激素释放激素（GnRH）具有同源性。迄今为止，ACP信号系统的功能仍不清楚。在本研究中，我们从分子上鉴定了编码埃及伊蚊ACP受体（ACPR）的完整开放阅读框，该受体跨越九个外显子并经历选择性剪接，产生三个转录变体。只有一个变体，AedaeACPR-I，产生推导的 577 个残基蛋白，包含视紫红质样 G 蛋白偶联受体特征的所有七个跨膜结构域。使用基于异源细胞培养的系统对 AedaeACPR-I 进行功能性去孤儿化，揭示了 AedaeACP 具有高度选择性和剂量依赖性的受体激活作用 (EC50 = 10.25 nM)。使用定量 RT-PCR 对所有胚胎后发育阶段的 AedaeACPR-I 和 AedaeACP 转录本水平进行分析，确定了成虫羽化后这两种转录本的富集。成年蚊子的组织特异性表达谱揭示了 AedaeACPR-I 受体转录本在中枢神经系统中的表达，包括腹部神经节内的显着富集。此外，AedaeACP 转录本在大脑和胸神经节中显着检测到。总的来说，这些结果表明 ACP 具有神经调节剂或神经递质作用，并表明这种神经肽可能在调节蜕皮后活动中发挥作用。
The recently discovered adipokinetic hormone/corazonin-related peptide (ACP) is an insect neuropeptide structurally intermediate between corazonin (CRZ) and adipokinetic (AKH) hormones, which all demonstrate homology to the vertebrate gonadotropin-releasing hormone (GnRH). To date, the function of the ACP signaling system remains unclear. In the present study, we molecularly identified the complete open reading frame encoding the Aedes aegypti ACP receptor (ACPR), which spans nine exons and undergoes alternative splicing giving rise to three transcript variants. Only a single variant, AedaeACPR-I, yielding a deduced 577 residue protein, contains all seven transmembrane domains characteristic of rhodopsin-like G protein-coupled receptors. Functional deorphanization of AedaeACPR-I using a heterologous cell culture-based system revealed highly-selective and dose-dependent receptor activation by AedaeACP (EC50 = 10.25 nM). Analysis of the AedaeACPR-I and AedaeACP transcript levels in all post-embryonic developmental stages using quantitative RT-PCR identified enrichment of both transcripts after adult eclosion. Tissue-specific expression profiling in adult mosquitoes reveals expression of the AedaeACPR-I receptor transcript in the central nervous system, including significant enrichment within the abdominal ganglia. Further, the AedaeACP transcript is prominently detected within the brain and thoracic ganglia. Collectively, these results indicate a neuromodulator or neurotransmitter role for ACP and suggest this neuropeptide may function in regulation of post-ecdysis activities.
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影响因子：
14.9
作者：
Dobson L;Reményi I;Tusnády GE
通讯作者：
Tusnády GE
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1073/pnas.0305291101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Kim, YJ;Spalovská-Valachová, I;Zitnan, D
通讯作者：
Zitnan, D
DOI：
10.1016/j.bbrc.2006.03.117
发表时间：
2006-05-26
影响因子：
3.1
作者：
Belmont, M;Cazzamali, G;Grimmelikhuijzen, CJP
通讯作者：
Grimmelikhuijzen, CJP
DOI：
10.1073/pnas.0809881106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Lindemans, Marleen;Liu, Feng;Schoofs, Liliane
通讯作者：
Schoofs, Liliane