Increased gelatinolytic activity in bronchoalveolar lavage fluid in stable lung transplant recipients.
Increased gelatinolytic activity in bronchoalveolar lavage fluid in stable lung transplant recipients.
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稳定肺移植受者的支气管肺泡灌洗液中的明胶分解活性增加。
DOI：
10.1164/ajrccm.156.6.9704044
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Ohar,JA
中科院分区：
文献类型：
--
作者：
Trello,CA;Williams,DA;Keller,CA;Crim,C;Webster,RO;Ohar,JA
蛋白水解酶在伴有肺实质重塑的各种炎性肺疾病的发病机制中发挥作用。为了评估炎性细胞和蛋白水解酶在肺移植后慢性同种异体移植物排斥反应发展中的作用，从临床稳定的肺移植（LT）受体（即，没有活动性感染或排斥的证据）、心脏移植（HT）受者和健康志愿者（NL）分析总白色血细胞（WBC）计数和分类细胞计数，沿着明胶溶解/IV型胶原溶解活性。与NL组相比，LT组显示总WBC计数、中性粒细胞计数和中性粒细胞百分比显著增加，证实存在炎症。此外，明胶酶谱显示，LT组与NL组相比，72和92 kD明胶酶的活性显着增加。中性粒细胞计数和蛋白水解活性的增加之间存在正相关。免疫抑制治疗不能解释这些发现，因为NL和HT对照组之间的细胞计数或蛋白水解活性没有显著差异。这些发现，连同那些与慢性肺同种异体移植物功能障碍的BALF中性粒细胞和胶原基质重塑的增加，共同表明，上调蛋白水解活性可能在肺移植后的慢性排斥反应中发挥作用。
Proteolytic enzymes have been proposed to play a role in the pathogenesis of various inflammatory pulmonary diseases accompanied by parenchymal remodeling. To assess the role of inflammatory cells and proteolytic enzymes in the development of chronic allograft rejection after lung transplantation, bronchoalveolar lavage fluid (BALF) samples from clinically stable lung transplant (LT) recipients (i.e., without evidence of active infection or rejection), heart transplant (HT) recipients, and healthy volunteers (NL) were analyzed for total white blood cell (WBC) count and differential cell count, along with gelatinolytic/type IV collagenolytic activity. The LT group displayed a significantly increased total WBC count, neutrophil count, and percent neutrophils compared with the NL group, confirming the presence of inflammation. Furthermore, gelatin zymography revealed a significant increase in activity of the 72 and 92 kD gelatinases in the LT group compared with the NL group. A positive correlation existed between neutrophil counts and the increase in proteolytic activity. Immunosuppressive therapy did not account for the findings, since no significant difference in cell counts or proteolytic activity existed between the NL and HT control groups. These findings, together with those of others that relate chronic lung allograft dysfunction to an increase in BALF neutrophils and collagen matrix remodeling, collectively indicate that up-regulated proteolytic activity may have a role in chronic rejection after lung transplantation.
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影响因子：
3.9
作者：
Y. Okada;S. Katsuda;Y. Okada;Isao Nakanishi
通讯作者：
Y. Okada;S. Katsuda;Y. Okada;Isao Nakanishi
DOI：
10.1016/s0021-9258(18)42873-6
发表时间：
1992-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Goldberg;A. Strongin;I. Collier;L. T. Genrich;B. Marmer
通讯作者：
G. Goldberg;A. Strongin;I. Collier;L. T. Genrich;B. Marmer
DOI：
10.1172/jci114867
发表时间：
1990-11
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
H. Welgus;E. J. Campbell;J D Cury;A. Z. Eisen;R. M. Senior;S. Wilhelm;G. I. Goldberg
通讯作者：
H. Welgus;E. J. Campbell;J D Cury;A. Z. Eisen;R. M. Senior;S. Wilhelm;G. I. Goldberg
微管中 GTP 水解伴随的结构变化：来自缓慢水解类似物鸟苷基-(α,β)-亚甲基二膦酸酯的信息。
DOI：
10.1083/jcb.128.1.117
发表时间：
1995-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hyman AA;Chrétien D;Arnal I;Wade RH
通讯作者：
Wade RH
对接受双肺和心肺移植的患者进行连续支气管肺泡灌洗研究。
影响因子：
0.9
作者：
J. Maurer;E. Gough;D. Chamberlain;G. Patterson;R. Grossman
通讯作者：
R. Grossman