Chemical modification of ribonucleic acid. A direct study by carbon-13 nuclear magnetic resonance spectroscopy.
Chemical modification of ribonucleic acid. A direct study by carbon-13 nuclear magnetic resonance spectroscopy.
复制标题
核糖核酸的化学修饰。
作者：
C. Chang;C. G. Lee
Direct study of the methylation of ribonucleic acid with methyl methanesulfonate by carbon-13 nuclear magnetic resonance spectroscopy has demonstrated the usefulness of this method in studying the chemical modification of biomacro-molecules and the interaction between nucleic acids and biologically active agents. This direct stable isotope method eliminated all tedious and questionable degradation processes for determining the reactive sites and the product distributions. Six methylated products, 7-methylguanosine, 1-methyladenosine, 3-methylcytidine, 1-methylguanosine, 3-methyluridine, and methyl phosphodiester, were identified by comparison with many model compounds and careful examination of spin-spin coupling and spin-lattice relaxation time. An extensive study of the interaction of phosphate buffer with methyl methansulfonate accounted for the sharp difference in the 13C spectra of the methylated RNA isolated from the reactions controlled by a pH-stat and phosphate buffer, respectively. The 13C-enriched agent significantly enhances the specificity and sensitivity of the method and provides better quantitative results.
通过碳 NMR 分析转移 RNA 的结构：从富含 13C 的纯化物种中解析单碳共振。
影响因子：
14.9
作者：
Agris,PF;Schmidt,PG
通讯作者：
Schmidt,PG