Pulsed near-infrared photoacoustic spectroscopy of blood
Pulsed near-infrared photoacoustic spectroscopy of blood
复制标题
血液脉冲近红外光声光谱
DOI：
10.1117/12.531159
复制
发表时间：
2004
影响因子：
--
通讯作者：
P. Beard
中科院分区：
文献类型：
--
作者：
J. Laufer;C. Elwell;D. Delpy;P. Beard
本研究的目的是使用脉冲近红外光声光谱法测定氧饱和度（SO2）的盐水悬浮液中的红细胞在体外。光声测量是在一个比色皿中进行的，该比色皿形成了一个较大回路的一部分，红细胞悬浮液通过该回路循环。使用膜式氧合器以2-3%至100%的步进增量改变红细胞悬液的氧饱和度，并且在每个增量处使用一氧化碳血氧计进行氧饱和度的独立测量。光学参量振荡器激光系统提供入射在比色皿上的近红外光谱（740- 1040 nm）中的多个波长的纳秒激发脉冲。使用宽带（15 MHz）法布里-珀罗聚合物膜换能器检测所产生的声信号。从作为波长的函数的声学信号确定光传输系数和振幅。然后，这些数据被用来计算的相对浓度的氧和脱氧血红蛋白，使用它们的已知的特定吸收系数和经验确定的波长依赖性的光散射的波长范围内调查。由此，与一氧化碳血氧计SO2测量值相比，悬浮液的氧饱和度以±5%的准确度导出。
The aim of this study was to use pulsed near infrared photoacoustic spectroscopy to determine the oxygen saturation (SO2) of a saline suspension of red blood cells in vitro. The photoacoustic measurements were made in a cuvette which formed part of a larger circuit through which the red blood cell suspension was circulated. Oxygen saturation of the red blood cell suspension was altered between 2-3% to 100% in step increments using a membrane oxygenator and at each increment an independent measurement of oxygen saturation was made using a co-oximeter. An optical parametric oscillator laser system provided nanosecond excitation pulses at a number of wavelengths in the near-infrared spectrum (740-1040nm) which were incident on the cuvette. The resulting acoustic signals were detected using a broadband (15MHz) Fabry-Perot polymer film transducer. The optical transport coefficient and amplitude were determined from the acoustic signals as a function of wavelength. These data were then used to calculate the relative concentrations of oxy- and deoxyhaemoglobin, using their known specific absorption coefficients and an empirically determined wavelength dependence of optical scattering over the wavelength range investigated. From this, the oxygen saturation of the suspension was derived with an accuracy of ±5% compared to the co-oximeter SO2 measurements.
影响因子：
1.9
作者：
Esenaliev, RO;Larina, IV;Prough, DS
通讯作者：
Prough, DS