The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties.
The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties.
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DOI：
10.1016/j.bbrep.2021.101003
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发表时间：
2021-07
影响因子：
2.7
通讯作者：
Tobiume K
中科院分区：
文献类型：
--
作者：
Uetsuki R;Higashikawa K;Okuda S;Yamakado N;Ishida F;Rizqiawan A;Ono S;Takechi M;Mizuta K;Shigeishi H;Kamata N;Tobiume K
低亲和力神经生长因子受体p75是人类上皮细胞的复层上皮干/祖标志物。我们发现，人鳞状细胞癌(SCC)细胞株OM-1在单层培养中表现出明显的p75簇状细胞，尤其是位于生长集落的中心。有p75簇的细胞被细胞角蛋白14和细胞角蛋白13表达的细胞包围，这些细胞定居在集落的外缘。OM-1细胞还能够在细胞悬浮培养中形成肿瘤球体，这一能力因抑制p75信号而减弱。有趣的是，我们还发现了来自OM-1不断增长的培养的p75阴性细胞群体，这些细胞重新承诺成为p75聚集性细胞。这些结果表明，具有上皮多层能力的SCC有可能利用p75依赖的分层上皮祖细胞特性来研究癌症的干性。鳞状细胞癌细胞系OM-1维持p75聚集性细胞群。单细胞来源的OM-1集落，以细胞为中心，维持分层的上皮层。OM-1干细胞具有p75依赖的上皮祖细胞和干细胞特性。
The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, a human squamous cell carcinoma (SCC) cell line, showed distinct cells with p75 cluster, especially located at the center of a growing colony in a monolayer culture. A cell with p75 cluster was surrounded by cytokeratin 14- and cytokeratin 13-expressing cells that settled at the outer margin of the colony. OM-1 cells were also capable of forming tumor spheres in a cell suspension culture, an ability which was attenuated by the inhibition of p75-signaling. Intriguingly, we also found a p75-negative cell population from a growing culture of OM-1 that re-committed to become p75-clustering cells. These results indicated the possibility that SCC with epithelial multi-layering capacity can exploit the p75-dependent stratified epithelial progenitor property for the cancer stemness. Squamous cell carcinoma cell line OM-1 maintained cell population with p75 cluster in the cell. Single cell-derived OM-1 colonies with the cell at centre maintained hierarchical stratified epithelial linages. OM-1 cells have p75-dependent epithelial progenitor and stemness properties.
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食管鳞状细胞癌中p75NTR阳性细胞的自我更新和化疗耐药性。
影响因子：
3.8
作者：
Huang, Sheng-Dong;Yuan, Yang;Liu, Xiao-Hong;Gong, De-Jun;Bai, Chen-Guang;Wang, Feng;Luo, Jun-Hui;Xu, Zhi-Yun
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Xu, Zhi-Yun
影响因子：
25
作者：
Yamashita, T;Tohyama, M
通讯作者：
Tohyama, M
影响因子：
5.7
作者：
Taki, M;Kamata, N;Nagayama, M
通讯作者：
Nagayama, M
影响因子：
11.2
作者：
Biddle, Adrian;Liang, Xiao;Mackenzie, Ian C.
通讯作者：
Mackenzie, Ian C.
影响因子：
2.6
作者：
Dowdall, Jayme R.;Sadow, Peter M.;Rajagopal, Jayaraj
通讯作者：
Rajagopal, Jayaraj