Elevated Serum Leptin Levels in Patients With Eosinophilic Chronic Rhinosinusitis.
Elevated Serum Leptin Levels in Patients With Eosinophilic Chronic Rhinosinusitis.
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DOI：
10.3389/fphar.2021.793607
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发表时间：
2021
影响因子：
5.6
通讯作者：
Fujieda S
中科院分区：
文献类型：
--
作者：
Imoto Y;Ueki S;Kato Y;Yoshida K;Morikawa T;Kimura Y;Kidoguchi M;Tsutsumiuchi T;Koyama K;Adachi N;Ito Y;Ogi K;Sakashita M;Yamada T;Schleimer RP;Takabayashi T;Fujieda S
背景：嗜酸性粒细胞性慢性鼻窦炎（ECRS）是慢性鼻窦炎伴鼻息肉（CRSwNP）的一种亚型，常与哮喘共病。值得注意的是，ECRS患者在手术切除后经常显示NP的高复发。瘦素是一种由脂肪细胞产生的激素，与气道炎症性疾病有关。然而，到目前为止，瘦素在ECRS中的作用尚未研究。 目的：探讨ECRS患者血清瘦素水平是否发生变化。 研究方法：本研究共纳入40例ECRS患者、15例非嗜酸性粒细胞CRS患者（非ECRS）和12例无CRS的个体（对照）。患者的血清瘦素水平进行了评估，并通过组织学评价的三个denelling与上皮表面下的细胞浸润区的嗜酸性粒细胞在其NP的数量进行了测量。最后，在体外用不同浓度的重组瘦素刺激从NP建立的鼻成纤维细胞培养物，以确定瘦素是否影响嗜酸性粒细胞趋化因子-3（趋化因子（C-C基序）配体26：26：CCL 26）表达。 结果如下：ECRS组和非ECRS组的血清瘦素水平均显著高于对照组（p < 0.0001 vs. ECRS; p < 0.05 vs.非ECRS）。此外，与非ECRS患者相比，ECRS患者显示出显著升高的血清瘦素水平（p < 0.001），尽管两组之间的体重指数没有差异。血清瘦素水平与外周血嗜酸性粒细胞比例（r = 0.3575，p < 0.01）和NP中嗜酸性粒细胞数量（r = 0.5109，p < 0.0001）相关。瘦素水平与嗜酸性粒细胞趋化因子-3mRNA表达呈正相关（r = 0.5374，p < 0.01）。最后，瘦素以瘦素受体依赖性方式显著增加体外从NP建立的鼻成纤维细胞中的嗜酸性粒细胞趋化因子-3表达（p < 0.05）。 结论：瘦素水平在ECRS患者中升高，并且可能促进和指示ECRS的严重程度以及全身性2型偏性炎症反应。结合，这些数据表明，循环瘦素可能发挥重要作用的发展嗜酸性粒细胞炎症的NP。
Background: Eosinophilic chronic sinusitis (ECRS) is a subtype of CRS with nasal polyps (CRSwNP) that is frequently comorbid with asthma. Notably, ECRS patients often show a high recurrence of NPs after surgical resection. Leptin is a hormone produced by adipocytes that has been implicated in airway inflammatory diseases. However, to date, the role of leptin in ECRS has not been investigated. Objective: To determine whether the serum levels of leptin are altered in patients with ECRS. Methods: In total, 40 patients with ECRS, 15 patients with non-eosinophilic CRS (non-ECRS), and 12 individuals without CRS (control) were included in this study. Patient’s serum leptin levels were assessed, and the number of eosinophils in their NPs were measured through a histological evaluation of the three densest areas with cellular infiltrate beneath the epithelial surface. Finally, nasal fibroblast cultures established from NPs were stimulated with varying concentrations of recombinant leptin in vitro to determine whether leptin affects eotaxin-3 (Chemokine (C-C motif) ligand 26 :26: CCL26) expression. Results: The serum leptin levels in both the ECRS and non-ECRS groups were significantly higher than those in the control subjects (p < 0.0001 vs. ECRS; p < 0.05 vs. non-ECRS). Furthermore, ECRS patients displayed significantly elevated serum leptin levels compared to non-ECRS patients (p < 0.001), although there was no difference in body mass index between the groups. Notably, serum leptin levels were correlated with the proportion of eosinophils in peripheral blood (r = 0.3575, p < 0.01) and the number of eosinophils in NPs (r = 0.5109, p < 0.0001). Serum leptin levels were also correlated with eotaxin-3 mRNA expression in NPs (r = 0.5374, p < 0.01). Finally, leptin significantly augmented eotaxin-3 expression in nasal fibroblasts established in vitro from NPs in a leptin receptor-dependent manner (p < 0.05). Conclusion: Leptin levels are elevated in ECRS patients and may both promote and indicate the severity of ECRS as well as systemic type 2-biased inflammatory responses. Combined, these data indicate that circulating leptin may play a significant role in the development of eosinophilic inflammation in NPs.
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影响因子：
76.2
作者：
Chupp, Geoffrey L.;Bradford, Eric S.;ten Brinke, Anneke
通讯作者：
ten Brinke, Anneke
影响因子：
--
作者：
Camargo, CA;Weiss, ST;Speizer, FE
通讯作者：
Speizer, FE
通过血清细胞因子谱的无偏聚类鉴定具有更严重疾病的哮喘的全身炎症内型
影响因子：
1.6
作者：
Liang Z;Liu L;Zhao H;Xia Y;Zhang W;Ye Y;Jiang M;Cai S
通讯作者：
Cai S
影响因子：
7.4
作者：
Dardeno, Tina A.;Chou, Sharon H.;Moon, Hyun-Seuk;Chamberland, John P.;Fiorenza, Christina G.;Mantzoros, Christos S.
通讯作者：
Mantzoros, Christos S.
影响因子：
8.8
作者：
Heiss, Christina N.;Manneras-Holm, Louise;Olofsson, Louise E.
通讯作者：
Olofsson, Louise E.