Novel effects of insulin secretagogues on capacitation of insulin release and survival of cultured pancreatic islets.
Novel effects of insulin secretagogues on capacitation of insulin release and survival of cultured pancreatic islets.
复制标题
胰岛素促分泌剂对胰岛素释放能力和培养胰岛存活的新影响。
DOI：
10.1152/ajpendo.1990.259.4.e548
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Moran,SM
中科院分区：
文献类型：
--
作者：
MacDonald,MJ;Fahien,LA;McKenzie,DI;Moran,SM
刺激新鲜胰岛释放胰岛素的药物被用于测试其使胰岛分泌胰岛素和支持组织培养中β细胞存活的能力。获能被定义为在促胰岛素浓度的促胰岛素剂存在下，培养24小时后释放胰岛素的能力。失去葡萄糖诱导的胰岛素释放的活胰岛在20 mM葡萄糖培养24小时后逐渐恢复胰岛素释放。生存被定义为重新获得葡萄糖诱导的胰岛素释放的能力。为了测量培养后胰岛素的释放，胰岛与各种分泌物在克雷布斯-林格缓冲液中孵育1小时。不同反应模式的例子包括：葡萄糖是唯一能促进葡萄糖诱导释放的促分泌剂。亮氨酸-、亮氨酸+谷氨酰胺-和甘油醛诱导的释放在没有促分泌剂的培养后仍然具有能力。高糖培养完全抑制亮氨酸诱导的释放。低葡萄糖（1mm）培养或高亮氨酸和谷氨酰胺培养均可消除葡萄糖诱导的释放。只有亮氨酸和谷氨酰胺能使琥珀酸单甲基诱导释放。除d -甘油醛外，包括促胰岛素亚葡萄糖（1mm）在内的所有药物均允许胰岛存活。因此，分泌物之间和内部的起始、获能和存活的代谢途径是不相同的。在能化方面，亮氨酸和葡萄糖呈反比关系。获能过程遵循一个时间过程，这表明它是由酶诱导调节的。
Agents that stimulate insulin release from fresh pancreatic islets were tested for their ability to capacitate pancreatic islets to secrete insulin and to support beta-cell survival in tissue culture. Capacitation was defined as the ability to release insulin after 24 h in culture in the presence of an insulinotropic concentration of a secretagogue. Viable islets that lose glucose-induced insulin release gradually regain it during culture for 24 h in 20 mM glucose. Survival was defined as the ability to regain glucose-induced insulin release. To measure insulin release after culture, islets were incubated with various secretagogues in Krebs-Ringer buffer for 1 h. Examples of the diverse patterns of responses included the following. Glucose was the only secretagogue that capacitated glucose-induced release. Leucine-, leucine plus glutamine-, and glyceraldehyde-induced release remained capacitated after culture with no secretagogue. Culture at high glucose completely inhibited leucine-induced release. Culture at low glucose (1 mM) or at both high leucine and glutamine abolished glucose-induced release. Only leucine and glutamine capacitated monomethyl succinate-induced release. All agents including subinsulinotropic glucose (1 mM), except D-glyceraldehyde, permitted islet survival. Thus the metabolic pathways for initiation, capacitation, and survival are not identical between and within secretagogues. There is a reciprocal relationship between leucine and glucose with respect to capacitation. Capacitation follows a time course, which suggests that it is regulated by enzyme induction.
登录
查看更多内容
服用口服避孕药的女性抗凝血酶 III 抑制和凝血酶生成加速
影响因子：
9.8
作者：
E. Kaulla;W. Droegemueller;N. Aoki;K. N. Kaulla
通讯作者：
K. N. Kaulla
晚期乳腺癌患者长期他莫昔芬治疗期间的激素变化。
影响因子：
3.5
作者：
I. Számel;B. Vincze;I. Hindy;I. Hermann;J. Borvendég;S. Eckhardt
通讯作者：
S. Eckhardt
辅助化疗和长期服用他莫昔芬对淋巴结阳性乳腺癌患者的内分泌影响。
影响因子：
11.2
作者：
V. Jordan;N. F. Fritz;D. Tormey
通讯作者：
D. Tormey
抗雌激素作用的生化药理学。
影响因子：
21.1
作者：
Jordan,VC
通讯作者：
Jordan,VC
他莫昔芬预防：预防胜于治疗——预防就是治疗？
影响因子：
3.6
作者：
V. Jordan
通讯作者：
V. Jordan