Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.
Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.
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DOI：
10.1038/ncomms5750
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发表时间：
2014-09-01
影响因子：
16.6
通讯作者：
You, Lingchong
中科院分区：
文献类型：
--
作者：
Dong, Peng;Maddali, Manoj V.;Srimani, Jaydeep K.;Thelot, Francois;Nevins, Joseph R.;Mathey-Prevot, Bernard;You, Lingchong
大量证据表明，E2 F转录因子活性的控制对于决定细胞周期进入和细胞增殖至关重要。然而，这种控制的确切决定因素，包括其他细胞周期调节活动的作用，尚未明确界定。在这里，认识到，个别监管组件的贡献可能会被掩盖的异质性群体的细胞，我们模型的潜在作用的个别组件一起使用的集成系统，以遵循E2 F动态在单细胞水平和真实的时间。这些分析表明，跨越E2 F积累的阈值幅度决定细胞周期的承诺。重要的是，我们发现Myc在调节振幅中是至关重要的，而细胞周期蛋白D/E活性对振幅几乎没有影响，但确实有助于调节E2 F激活的持续时间，从而影响细胞周期进展的步伐。 转录因子E2 F对决定细胞增殖至关重要。通过在整个细胞周期中监测单细胞中的E2 F活性，Dong等人提供了Myc和G1细胞周期蛋白/CDKs调节E2 F时间动力学的不同方面的证据，导致不同的表型输出。
A body of evidence has shown that the control of E2F transcription factor activity is critical for determining cell cycle entry and cell proliferation. However, an understanding of the precise determinants of this control, including the role of other cell-cycle regulatory activities, has not been clearly defined. Here, recognizing that the contributions of individual regulatory components could be masked by heterogeneity in populations of cells, we model the potential roles of individual components together with the use of an integrated system to follow E2F dynamics at the single-cell level and in real time. These analyses reveal that crossing a threshold amplitude of E2F accumulation determines cell cycle commitment. Importantly, we find that Myc is critical in modulating the amplitude, whereas cyclin D/E activities have little effect on amplitude but do contribute to the modulation of duration of E2F activation, thereby affecting the pace of cell cycle progression. The transcription factor E2F is critical for determining cell proliferation. By monitoring E2F activity in single cells throughout the cell cycle, Dong et al. provide evidence that Myc and G1 cyclin/CDKs regulate different aspects of E2F temporal dynamics, resulting in distinct phenotypic outputs.
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影响因子：
21.3
作者：
Campaner, Stefano;Doni, Mirko;Amati, Bruno
通讯作者：
Amati, Bruno
Ankyrin-G的半胱氨酸70已被S-膜酰化化，并且是膜结构域组装中Ankyrin-G的功能所必需的。
DOI：
10.1074/jbc.m112.417501
发表时间：
2012-12-21
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
He M;Jenkins P;Bennett V
通讯作者：
Bennett V
影响因子：
64.5
作者：
Delmore JE;Issa GC;Lemieux ME;Rahl PB;Shi J;Jacobs HM;Kastritis E;Gilpatrick T;Paranal RM;Qi J;Chesi M;Schinzel AC;McKeown MR;Heffernan TP;Vakoc CR;Bergsagel PL;Ghobrial IM;Richardson PG;Young RA;Hahn WC;Anderson KC;Kung AL;Bradner JE;Mitsiades CS
通讯作者：
Mitsiades CS
影响因子：
64.5
作者：
Geng, Y;Yu, QY;Sicinski, P
通讯作者：
Sicinski, P
影响因子：
64.8
作者：
JOHNSON, DG;SCHWARZ, JK;NEVINS, JR
通讯作者：
NEVINS, JR