Expression of proopiomelanocortin peptides in human dermal microvascular endothelial cells: evidence for a regulation by ultraviolet light and interleukin-1.
Expression of proopiomelanocortin peptides in human dermal microvascular endothelial cells: evidence for a regulation by ultraviolet light and interleukin-1.
复制标题
阿黑皮质素原肽在人真皮微血管内皮细胞中的表达：紫外线和白介素-1 调节的证据。
DOI：
10.1046/j.1523-1747.2000.00174.x
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Luger,TA
中科院分区：
文献类型：
--
作者：
Scholzen,TE;Kalden,DH;Brzoska,T;Fisbeck,T;Fastrich,M;Schiller,M;Böhm,M;Schwarz,T;Armstrong,CA;Ansel,JC;Luger,TA
Proopiomelanocortin peptides such as α-melanocyte-stimulating hormone and adrenocorticotropin are expressed in the epidermal and dermal compartment of the skin after noxious stimuli and are recognized as modulators of immune and inflammatory reactions. Human dermal microvascular endothelial cells mediate leukocyte–endothelial interactions during cutaneous inflammation by the expression of cellular adhesion molecules and cytokines such as interleukin-1. This study addresses the hypothesis that human dermal microvascular endothelial cells express both proopiomelanocortin and prohormone convertases, which are required to generate proopiomelanocortin peptides. Semiquantitative reverse transcriptase polymerase chain reaction and northern blot studies revealed a constitutive expression of proopiomelanocortin mRNA by human dermal microvascular endothelial cellsin vitrothat was time- and concentration-dependently upregulated by interleukin-1β. Furthermore, irradiation of human dermal microvascular endothelial cells with ultraviolet A1 (30 J per cm2) or ultraviolet B (12.5 mJ per cm2) enhanced proopiomelanocortin expression as well as the production and release of the proopiomelanocortin peptides adrenocorticotropin and α-melanocyte-stimulating hormone. In addition to proopiomelanocortin, prohormone convertase 1 mRNA expression was detected by reverse transcriptase polymerase chain reaction in unstimulated human dermal microvascular endothelial cells and was augmented after exposure to α-melanocyte- stimulating hormone, interleukin-1β, or irradiation with ultraviolet. These findings demonstrate that human dermal microvascular endothelial cells express proopiomelanocortin and prohormone convertase 1 required for the generation of adrenocorticotropin. Additionally, human dermal microvascular endothelial cells express mRNA for the prohormone convertase 2 binding protein 7B2. Taken together these findings indicate that human dermal microvascular endothelial cells upon stimulation express both proopiomelanocortin and prohormone convertases required for the generation of α-melanocyte-stimulating hormone. As proopiomelanocortin peptides were found to regulate the production of human dermal microvascular endothelial cell cytokines and adhesion molecules and to have a variety of anti-inflammatory properties these peptides may significantly contribute to the modulation of skin inflammation.
登录
查看更多内容
阿黑皮质素原衍生肽可诱导人单核细胞产生 IL-10。
影响因子：
4.4
作者：
R. Bhardwaj;A. Schwarz;E. Becher;K. Mahnke;Y. Aragane;T. Schwarz;T. Luger
通讯作者：
T. Luger
神经肽 α-MSH 在人类 HIV 感染和其他疾病中的作用
DOI：
--
发表时间：
1998
期刊：
影响因子：
--
作者：
A. Catania;L. Airaghi;L. Garofalo;M. Cutuli;J. M. Lipton
通讯作者：
J. M. Lipton
皮肤恶性黑色素瘤和良性黑素细胞痣中α-黑素细胞刺激激素、促肾上腺皮质激素和β-内啡肽的免疫反应性
影响因子：
10.3
作者：
Nagahama;Funasaka;Fernandez‐Frez;Ohashi;Chakraborty;Ueda;Ichihashi
通讯作者：
Ichihashi
促肾上腺皮质激素和促黑素激素的免疫抑制作用及其在人类免疫缺陷病毒感染中的可能意义。
DOI：
10.1073/pnas.89.2.782
发表时间：
1992
影响因子：
11.1
作者：
Smith,EM;HughesJr,TK;Hashemi,F;Stefano,GB
通讯作者：
Stefano,GB
结节型皮肤恶性黑色素瘤中 α、β 和 γ3 黑色素细胞刺激激素表达的免疫组织化学证据
DOI：
--
发表时间：
1995
期刊：
影响因子：
--
作者：
P. Liu;O. Johansson
通讯作者：
O. Johansson