An energy landscape approach to understanding variety and robustness in tissue morphogenesis
An energy landscape approach to understanding variety and robustness in tissue morphogenesis
复制标题
理解组织形态发生的多样性和稳健性的能量景观方法
DOI：
10.1007/s10237-019-01222-5
复制
发表时间：
2020
影响因子：
3.5
通讯作者：
Yasuhiro Inoue and Taiji Adachi
中科院分区：
文献类型：
--
作者：
Hironori Takeda;Yoshitaka Kameo;Yasuhiro Inoue and Taiji Adachi
在发育的形态发生过程中，多细胞组织因时空调节的细胞活动（例如细胞增殖和收缩）诱导的机械力而变形。由于多细胞活动的不同时空组合和顺序，形成了不同的形态。尽管形态发生具有变异的潜力，但它是一个令人惊讶的稳健过程，其中即使在多细胞活动的时空波动下，也可重复地形成性质相似的形态。为了理解组织形态发生的这些基本特征，包括各种形态的共存和形态发生过程的鲁棒性，在本研究中，我们提出了一种从力学角度捕捉形态发生整体视图的新方法。这种方法将使能量景观可视化，其中包括由可接受的细胞活动历史引起的形态发生过程。该方法用于研究具有曲率的片状组织的形态发生，该组织根据生长和收缩的历史变形为凹形或凸形形态。能量景观中山谷的分叉产生了质的不同形态。稳定状态附近山谷的深度和陡度代表了对多细胞活动波动的稳健程度。此外，作为一个现实的例子，我们展示了这种方法在肠绒毛形成初始阶段观察到的管腔折叠的应用。这种方法将有助于理解各种形态形成的机制以及组织如何可重复地实现特定形态。
During morphogenesis in development, multicellular tissues deform by mechanical forces induced by spatiotemporally regulated cellular activities, such as cell proliferation and constriction. Various morphologies are formed because of various spatiotemporal combinations and sequences of multicellular activities. Despite its potential to variations, morphogenesis is a surprisingly robust process, in which qualitatively similar morphologies are reproducibly formed even under spatiotemporal fluctuation of multicellular activities. To understand these essential characteristics of tissue morphogenesis, which involves the coexistence of various morphologies and robustness of the morphogenetic process, in this study, we propose a novel approach to capture the overall view of morphogenesis from mechanical viewpoints. This approach will enable visualization of the energy landscape, which includes morphogenetic processes induced by admissible histories of cellular activities. This approach was applied to investigate the morphogenesis of a sheet-like tissue with curvature, where it deformed to a concave or convex morphology depending on the history of growth and constriction. Qualitatively different morphologies were produced by bifurcation of the valley in the energy landscape. The depth and steepness of the valley near the stable states represented the degree of robustness to fluctuations of multicellular activities. Furthermore, as a realistic example, we showed an application of this approach to luminal folding observed in the initial stage of intestinal villus formation. This approach will be helpful to understand the mechanism of how various morphologies are formed and how tissues reproducibly achieve specific morphologies.
登录
查看更多内容
影响因子：
2
作者：
Conte, Vito;Munoz, Jose J.;Miodownik, Mark
通讯作者：
Miodownik, Mark
影响因子：
2.4
作者：
Menzel A;Kuhl E
通讯作者：
Kuhl E
影响因子：
2.7
作者：
Huang J;Rajagopal R;Liu Y;Dattilo LK;Shaham O;Ashery-Padan R;Beebe DC
通讯作者：
Beebe DC
影响因子：
3.3
作者：
Royou, A;Field, C;Karess, R
通讯作者：
Karess, R
影响因子：
2
作者：
GOODWIN, BC;KAUFFMAN, S;MURRAY, JD
通讯作者：
MURRAY, JD