Historical BCG vaccination combined with drug treatment enhances inhibition of mycobacterial growth ex vivo in human peripheral blood cells
Historical BCG vaccination combined with drug treatment enhances inhibition of mycobacterial growth ex vivo in human peripheral blood cells
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历史上的卡介苗疫苗接种与药物治疗相结合增强了对人外周血细胞中分枝杆菌离体生长的抑制
DOI：
10.1038/s41598-019-41008-4
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发表时间：
2019
影响因子：
4.6
通讯作者：
Prabowo S
中科院分区：
文献类型：
--
作者：
Prabowo S
结核病（TB）是全球主要的传染性死亡原因。药物治疗和疫苗接种，特别是卡介苗，仍然是控制结核病的主要战略。随着耐药性的出现，有人提出，结核病疫苗接种与药物治疗相结合可能会提供更大的治疗价值。我们实施了anex活菌生长抑制试验（MGIA），以区分历史上接种卡介苗的人类志愿者的疫苗应答，并评估在抗生素异烟肼（INH）和利福平（RIF）存在下，疫苗介导的免疫应答对分枝杆菌杀灭效果的贡献，试图将该试验开发为治疗性TB疫苗的筛选工具。BCG疫苗接种显著增强了INH控制体内分枝杆菌生长的能力。当外周血单个核细胞（PBMC）与INH共培养时，BCG接种组显示出更高的IFN-γ和IP-10产生，与RIF共培养期间的趋势相似。观察到IFN-γ+和TNF-α+ CD 3 − CD 4 − CD 8 −细胞的频率较高，表明自然杀伤（NK）细胞在BCG疫苗接种和INH之间的联合效应中起作用。综上所述，我们的数据表明INH的疗效可以在历史BCG疫苗接种后增强，这支持了先前观察和动物研究的结果。
Tuberculosis (TB) is a leading infectious cause of death globally. Drug treatment and vaccination, in particular with Bacillus Calmette-Guérin (BCG), remain the main strategies to control TB. With the emergence of drug resistance, it has been proposed that a combination of TB vaccination with pharmacological treatment may provide a greater therapeutic value. We implemented anex vivomycobacterial growth inhibition assay (MGIA) to discriminate vaccine responses in historically BCG-vaccinated human volunteers and to assess the contribution of vaccine-mediated immune response towards the killing effect of mycobacteria in the presence of the antibiotics isoniazid (INH) and rifampicin (RIF), in an attempt to develop the assay as a screening tool for therapeutic TB vaccines. BCG vaccination significantly enhanced the ability of INH to control mycobacterial growthex vivo. The BCG-vaccinated group displayed a higher production of IFN-γ and IP-10 when peripheral blood mononuclear cells (PBMC) were co-cultured with INH, with a similar trend during co-culture with RIF. A higher frequency of IFN-γ+and TNF-α+CD3−CD4−CD8−cells was observed, suggesting the contribution of Natural Killer (NK) cells in the combined effect between BCG vaccination and INH. Taken together, our data indicate the efficacy of INH can be augmented following historical BCG vaccination, which support findings from previous observational and animal studies.
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DOI：
10.4049/jimmunol.1102710
发表时间：
2012-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Horowitz A;Hafalla JC;King E;Lusingu J;Dekker D;Leach A;Moris P;Cohen J;Vekemans J;Villafana T;Corran PH;Bejon P;Drakeley CJ;von Seidlein L;Riley EM
通讯作者：
Riley EM
影响因子：
3.1
作者：
Rosenwax L;Spilsbury K;McNamara BA;Semmens JB
通讯作者：
Semmens JB
流行病学：死敌
影响因子：
64.8
作者：
T. Paulson
通讯作者：
T. Paulson
影响因子：
2.2
作者：
Babalik, Aylin;Mannix, Sharyn;Menzies, Dick
通讯作者：
Menzies, Dick
影响因子：
7.7
作者：
Mangtani, Punam;Nguipdop-Djomo, Patrick;Rodrigues, Laura C.
通讯作者：
Rodrigues, Laura C.