Antigen-specific IL-2 secretion correlates with NK cell responses after immunization of Tanzanian children with the RTS,S/AS01 malaria vaccine.
Antigen-specific IL-2 secretion correlates with NK cell responses after immunization of Tanzanian children with the RTS,S/AS01 malaria vaccine.
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DOI：
10.4049/jimmunol.1102710
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发表时间：
2012-05-15
期刊：
影响因子：
--
通讯作者：
Riley EM
中科院分区：
文献类型：
--
作者：
Horowitz A;Hafalla JC;King E;Lusingu J;Dekker D;Leach A;Moris P;Cohen J;Vekemans J;Villafana T;Corran PH;Bejon P;Drakeley CJ;von Seidlein L;Riley EM
RTS，S/AS 01是一种针对恶性疟原虫红细胞前期阶段的疫苗，正在进行临床试验。我们报告了一项IIb期RTS，S/AS 01 E试验中坦桑尼亚儿童对RTS，S -B肝炎表面抗原（HBs）和恶性疟原虫环子孢子（CS）蛋白组分抗原的细胞免疫应答分析。RTS，S/AS 01 E疫苗对HBs肽的T细胞IFN-γ、CD 69和CD 25应答比对照强，表明RTS，S增强了预先存在的HBs应答。RTS、S疫苗接种增强了T细胞CD 69和CD 25对CS和CS特异性分泌的IL-2的应答。重要的是，超过50%的肽诱导的IFN-γ+淋巴细胞是NK细胞，并且NK细胞CD 69对HBs肽的应答的幅度与分泌的IL-2浓度相关。CD 69和CD 25表达和IL-2分泌可能代表RTS、S诱导的CS特异性T细胞的敏感标志物。T细胞来源的IL-2在RTS、S疫苗接种个体中增强NK细胞活化的潜力，以及这与保护的相关性，需要进一步探索。
RTS,S/AS01, a vaccine targeting pre-erythrocytic stages of Plasmodium falciparum, is undergoing clinical trials. We report an analysis of cellular immune response to component antigens of RTS,S – hepatitis B surface antigen (HBs) and P. falciparum circumsporozoite (CS) protein – among Tanzanian children in a Phase IIb RTS,S/AS01E trial. RTS,S/AS01 E vaccinees make stronger T cell IFN-γ, CD69 and CD25 responses to HBs peptides than do controls, indicating that RTS,S boosts pre-existing HBs responses. T cell CD69 and CD25 responses to CS and CS-specific secreted IL-2, were augmented by RTS,S vaccination. Importantly, more than 50% of peptide-induced IFN-γ+ lymphocytes were NK cells and the magnitude of the NK cell CD69 response to HBs peptides correlated with secreted IL-2 concentration. CD69 and CD25 expression and IL-2 secretion may represent sensitive markers of RTS,S-induced, CS-specific T cells. The potential for T cell-derived IL-2 to augment NK cell activation in RTS,S-vaccinated individuals, and the relevance of this for protection, needs to be explored further.
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影响因子：
5.5
作者：
Berthoud, Tamara K.;Fletcher, Helen;Todryk, Stephen M.
通讯作者：
Todryk, Stephen M.
影响因子：
56.3
作者：
Olotu, Ally;Lusingu, John;Bejon, Philip
通讯作者：
Bejon, Philip
DOI：
10.1007/978-1-60761-362-6_29
发表时间：
2010-01-01
期刊：
NATURAL KILLER CELL PROTOCOLS
影响因子：
--
作者：
Horowitz, Amir;Riley, Eleanor M.
通讯作者：
Riley, Eleanor M.
影响因子：
158.5
作者：
Abdulla, Salim;Oberholzer, Rolf;Tanner, Marcel
通讯作者：
Tanner, Marcel
影响因子：
4.4
作者：
Artavanis-Tsakonas, K;Eleme, K;Riley, EM
通讯作者：
Riley, EM