Reproducibility of serologic assays for influenza virus A (H5N1).
Reproducibility of serologic assays for influenza virus A (H5N1).
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DOI：
10.3201/eid1508.081754
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发表时间：
2009-08
影响因子：
11.8
通讯作者：
Wood JM
中科院分区：
文献类型：
--
作者：
Stephenson I;Heath A;Major D;Newman RW;Hoschler K;Junzi W;Katz JM;Weir JP;Zambon MC;Wood JM
当使用标准抗体时，实验室之间的进化枝1病毒结果更加一致。血凝抑制（HI）和中和用于评估甲型流感病毒（H5 N1）的疫苗;然而，标准化差导致实验室间结果的差异。从接种进化枝1A/Vietnam/1194/2004疫苗的人的合并血浆制备候选抗体标准品（07/150）。为了检测人和羊的抗血清，15个实验室使用HI和中和和再结合剂A/Vietnam/1194/2004、A/turkey/Turkey/1/2005（进化枝2.2）和A/Anhui/1/2005（进化枝2.3.4）病毒。两种检测均观察到实验室间变异，但当相对于07/150表示滴度时，A/Vietnam/1194/2004的总体几何变异系数百分比从HI的125%降至61%，中和从183%降至81%。进化枝2抗原缺乏降低的变异性表明需要进化枝特异性标准品。绵羊抗血清作为标准品不能可靠地降低变异性。世界卫生组织已将07/150确立为针对进化枝1亚型H5的抗体的国际标准，并且具有1，000 IU/ml的指定效力。
Results for clade 1 viruses were more consistent among laboratories when a standard antibody was used. Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization and reassortant A/Vietnam/1194/2004, A/turkey/Turkey/1/2005 (clade 2.2), and A/Anhui/1/2005 (clade 2.3.4) viruses. Interlaboratory variation was observed for both assays, but when titers were expressed relative to 07/150, overall percentage geometric coefficient of variation for A/Vietnam/1194/2004 was reduced from 125% to 61% for HI and from 183% to 81% for neutralization. Lack of reduced variability to clade 2 antigens suggested the need for clade-specific standards. Sheep antiserum as a standard did not reliably reduce variability. The World Health Organization has established 07/150 as an international standard for antibody to clade 1 subtype H5 and has an assigned potency of 1,000 IU/ampoule.
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影响因子：
168.9
作者：
Nicholson, KG;Colegate, AE;Zambon, MC
通讯作者：
Zambon, MC
影响因子：
5.5
作者：
Gulati, U;Kumari, K;Air, GM
通讯作者：
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影响因子：
9.4
作者：
OKUNO, Y;TANAKA, K;UEDA, S
通讯作者：
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影响因子：
1.7
作者：
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通讯作者：
Heath, A
影响因子：
5.5
作者：
WOOD, JM;GAINESDAS, RE;CHAKRAVERTY, P
通讯作者：
CHAKRAVERTY, P