Characterizations of H4 avian influenza viruses isolated from ducks in live poultry markets and farm in Shanghai.
Characterizations of H4 avian influenza viruses isolated from ducks in live poultry markets and farm in Shanghai.
复制标题
上海活禽市场和养殖场鸭子H4型禽流感病毒特征分析
DOI：
10.1038/srep37843
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发表时间：
2016-11-29
影响因子：
4.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Shi Y;Cui H;Wang J;Chi Q;Li X;Teng Q;Chen H;Yang J;Liu Q;Li Z
H4禽流感病毒是鸟类中最流行的流感病毒亚型之一。H4亚型禽流感病毒在中国家禽中的进化和致病性尚不清楚。本研究对从鸭场和活禽市场分离的8株H4亚型禽流感病毒进行了特征分析。系统发育分析表明，这些菌株分为两个组的欧亚血统。从某鸭场分离到的MH-2/H4 N6病毒的8个基因与3株LPM中的H4 N6病毒有密切的亲缘关系，提示鸭场与LPM之间可能存在禽流感病毒的关联。此外，在LPM中分离的另外两种H4 N6病毒的HA、NA、PB 2、NP和NS基因与MH-2/H4 N6的基因聚类。然而，其余的基因与其他亚系的关系更密切，这表明MH-2/H4 N6起源的病毒与LPM中的其他病毒重新组合。所有的H4病毒复制在小鼠肺没有事先适应和所有病毒复制和鸭之间传播。29-1/H4 N2、MH-2/H4 N6和420-2/H4 N6病毒引起感染鸭的全身感染。然而，大多数病毒在鸡中不适应。目前的研究结果表明，禽流感病毒之间的潜在相关性LPM和农场，并建议在LPM中的AIV的积极监测是必要的。
H4 avian influenza virus is one of the most prevalent influenza virus subtypes in birds. The evolution and pathogenicity of H4 AIV in domestic birds of China remain largely unclear. In the present study, a total of eight H4 AIV strains isolated in duck farm and live poultry markets (LPM) were characterized. Phylogenetic analysis indicated that these strains are divided into two groups in the Eurasian lineage. Eight genes of MH-2/H4N6 isolated from a duck farm were closely related to three H4N6 viruses from LPM, suggesting a potential AIV link between farms and LPMs. Additionally, the HA, NA, PB2, NP, and NS genes of two other H4N6 viruses isolated in LPM clustered with that of MH-2/H4N6. However, the remaining genes were more closely related to other sublineages, suggesting that MH-2/H4N6-originated viruses reassorted with other viruses in LPM. All H4 viruses replicated in mouse lungs without prior adaptation and all viruses replicated and transmitted among ducks. 29-1/H4N2, MH-2/H4N6, and 420-2/H4N6 viruses caused systemic infection in infected ducks. However, most of the viruses were not adapted in chickens. The present results indicate a potential correlation of AIV between LPMs and farms and suggest that active surveillance of AIV in LPM is warranted in China.
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影响因子：
5.4
作者：
Liang L;Deng G;Shi J;Wang S;Zhang Q;Kong H;Gu C;Guan Y;Suzuki Y;Li Y;Jiang Y;Tian G;Liu L;Li C;Chen H
通讯作者：
Chen H
DOI：
10.1007/978-1-4939-0758-8_6
发表时间：
2014-01-01
期刊：
ANIMAL INFLUENZA VIRUS, SECOND EDITION
影响因子：
--
作者：
Spackman, Erica
通讯作者：
Spackman, Erica
影响因子：
3.1
作者：
Lee, MS;Chang, PC;Shieh, HK
通讯作者：
Shieh, HK
影响因子：
6.4
作者：
Wang, Chengmin;Wang, Jing;He, Hongxuan
通讯作者：
He, Hongxuan
影响因子：
3.7
作者：
Kayali G;Barbour E;Dbaibo G;Tabet C;Saade M;Shaib HA;Debeauchamp J;Webby RJ
通讯作者：
Webby RJ