Characterization of Human Mononuclear Cells after Positive Selection with Immunomagnetic Particles
Characterization of Human Mononuclear Cells after Positive Selection with Immunomagnetic Particles
复制标题
免疫磁性颗粒阳性选择后人单核细胞的表征
DOI：
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发表时间：
1986
影响因子：
3.7
通讯作者：
J. Ugelstad
中科院分区：
文献类型：
--
作者：
T. Lea;E. Smeland;S. Funderud;F. Vartdal;C. Davies;K. Beiske;J. Ugelstad
我们研究了使用磁性单分散聚合物颗粒对人外周血单个核细胞群体进行阳性选择的可能性。通过仔细滴定颗粒和细胞之间的比例，我们成功地分离出许多细胞群，这些细胞群可以随后在体外培养用于功能研究。该过程的成功部分取决于用于致敏细胞的单克隆抗体的特性。如果这些抗体不与参与活化信号转导的膜结构一起读取，则可以在单个分级分离步骤中回收高度纯化的静止细胞群。在大多数情况下，通过过夜培养，颗粒将从分离的细胞分离，然后可以通过合适的磁体从系统中除去颗粒。T淋巴细胞、I淋巴细胞亚群和B淋巴细胞已经以这种方式分离，并在各种功能测定系统中进行了研究。与阴性选择后获得的细胞的比较清楚地证明了该技术的有用性，特别是如果为其选择的膜标记物不直接参与活化过程。
We have investigated the possibility to employing magnetic monodisperse polymer particles for positive selection of human peripheral blood mononuclear cell populations. By carefully titrating the ratio between particles and cells we succeeded in isolating a number of cell populations that could be cultivated subsequently in vitro for functional studies. The success of the procedure is partly dependent on the properties of the monoclonal antibodies used to sensitize the cells. Provided these antibodies do not read with membrane structures involved in the transduction of activating signals, highly purified, quiescent cell populations can be recovered in a single fractionation step. In most instances panicles will detach from the isolated cells by overnight culture, and the particles can then be removed from the system by a suitable magnet. T lymphocytes, subpopulations of I lymphocytes, and B lymphocytes have been isolated in this way and studied in a variety of functional assay systems. Comparison with cells obtained after negative selection clearly demonstates the usefulness of this technique, especially if the membrane marker selected for it is not directly engaged in the activation processes.
影响因子：
4.4
作者：
P. Stashenko;L. Nadler;R. Hardy;S. Schlossman
通讯作者：
P. Stashenko;L. Nadler;R. Hardy;S. Schlossman
一种使用单克隆抗体分离功能完整的人 T 淋巴细胞的快速方法。
DOI：
10.1016/0090-1229(81)90214-2
发表时间：
1981
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Reinherz,EL;Penta,AC;Hussey,RE;Schlossman,SF
通讯作者：
Schlossman,SF
抗 Leu-2 和抗 Leu-3 抗体阻断人 T 淋巴细胞功能：增殖和抑制的差异抑制。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Engleman,EG;Benike,CJ;Metzler,C;Gatenby,PA;Evans,RL
通讯作者：
Evans,RL