Zinc Detection in Serum by Anodic Stripping Voltammetry on Microfabricated Bismuth Electrodes.
Zinc Detection in Serum by Anodic Stripping Voltammetry on Microfabricated Bismuth Electrodes.
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DOI：
10.1002/elan.201200530
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发表时间：
2013-02
期刊：
影响因子：
3
通讯作者：
Papautsky, Ian
中科院分区：
文献类型：
--
作者：
Jothimuthu, Preetha;Wilson, Robert A.;Herren, Josi;Pei, Xing;Kang, Wenjing;Daniels, Rodney;Wong, Hector;Beyette, Fred;Heineman, William R.;Papautsky, Ian
锌（Zn）的体内平衡是免疫系统正常运转所必需的。危重患者往往表现出血清锌浓度降低，可能受益于锌补充作为治疗策略。然而，传统的监测锌的方法既耗时又昂贵。本文报道了用阳极溶出伏安法（ASV）在微制造电化学电池的铋电极上检测Zn的方法。用循环伏安法研究电沉积铋膜电极的工作电位窗口，用方波ASV法测定醋酸缓冲液和血清中的锌。对预富集电位、预富集时间和缓冲液pH等关键检测条件进行了优化。在5 μM ~ 50μM Zn的生理相关范围内，用pH 6醋酸缓冲液对传感器进行了成功校准，并显示出定义明确且高度可重复的峰。该传感器用于测定HCl消化的血清中锌的含量。结果表明，与传统的光谱方法相比，可以用更小的样品体积（μL vs. μL）和更快的周转时间（小时vs.天）检测血清中的锌。
Zinc (Zn) homeostasis is required for a functional immune system. Critically ill patients often exhibit decreased Zn serum concentrations and could potentially benefit from Zn supplementation as a therapeutic strategy. However, the conventional approaches to monitoring Zn are time consuming and costly. This work reports on detection of Zn by anodic stripping voltammetry (ASV) on bismuth electrodes in a microfabricated electrochemical cell. The working potential window of the electrodeposited bismuth film electrode was investigated by cyclic voltammetry, while square wave ASV was used for measuring Zn in acetate buffer and blood serum. Conditions critical to sensing, such as preconcentration potential, preconcentration time, and buffer pH, were optimized for Zn detection. The sensor was successfully calibrated with pH 6 acetate buffer in the physiologically-relevant range of 5 μM to 50μM Zn and exhibited well-defined and highly repeatable peaks. The sensor was used to demonstrate measurement of Zn in blood serum digested in HCl. The results of this work show that Zn detection in serum is possible with smaller sample volumes (μL vs. μL) and faster turnaround time (hours vs. days) as compared with the conventional spectroscopic methods.
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影响因子：
6.1
作者：
Guo, ZY;Feng, F;Jaffrezic-Renault, N
通讯作者：
Jaffrezic-Renault, N
影响因子：
6.2
作者：
Lu, TH;Huang, JF;Sun, IW
通讯作者：
Sun, IW
影响因子：
2.8
作者：
Jothimuthu, Preetha;Wilson, Robert A.;Herren, Josi;Haynes, Erin N.;Heineman, William R.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
8.4
作者：
Desmond, D;Lane, B;Glennon, JD
通讯作者：
Glennon, JD
影响因子：
6.2
作者：
Kruusma, J;Banks, CE;Compton, RG
通讯作者：
Compton, RG