Expression Profile of microRNAs during Development of the Hypopharyngeal Gland in Honey Bee, Apis mellifera.
Expression Profile of microRNAs during Development of the Hypopharyngeal Gland in Honey Bee, Apis mellifera.
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蜜蜂下咽腺发育过程中 microRNA 的表达谱
DOI：
10.3390/ijms232112970
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发表时间：
2022-10-26
影响因子：
5.6
通讯作者：
Wang, Zilong
中科院分区：
文献类型：
--
作者：
Qin, Kaixin;Cheng, Fuping;Pan, Luxia;Wang, Zilong
下咽腺是蜜蜂分泌蜂王浆的重要器官，其分泌活性随工蜂年龄的变化而变化。然而，到目前为止，下咽腺发育的调控机制尚不清楚。在这里，通过小RNA测序研究了新出现的工蚁、护士和觅食者下咽腺中mirna的表达谱。在这三个阶段，已经鉴定出81个已知的mirna和135个新的mirna。共有85个mirna在不同发育阶段表现出表达差异，它们的靶基因预计在1到10个以上。许多差异表达的mirna和靶基因与生长发育或细胞凋亡有关。此外，双荧光素酶报告基因检测证实，novel-miR-11直接靶向LOC410685（失活酪氨酸蛋白激酶7）和LOC725318（未表征蛋白）的3 ' -非翻译区。这些结果表明，mirna广泛参与蜜蜂下咽腺的发育调控。
The hypopharyngeal gland is an important organ for honey bees to secrete royal jelly, and its secretory activity varies with the age of workers. However, by now, the regulation mechanism of hypopharyngeal gland development is still unclear. Here, the expression profiles of miRNAs in the hypopharyngeal gland of newly emerged workers, nurses, and foragers were investigated via small RNA sequencing. From these three stages, 81 known miRNAs and 135 novel miRNAs have been identified. A total of 85 miRNAs showed expression differences between different development stages, and their target genes were predicted to range from 1 to more than 10. Many of the differentially expressed miRNAs and target genes are related to growth and development or apoptosis. Moreover, dual-luciferase-reporter assays verified that novel-miR-11 directly targets the 3′-untranslated regions of LOC410685 (inactive tyrosine-protein kinase 7) and LOC725318 (uncharacterized protein). These results suggested that miRNAs were widely involved in the developmental regulation of the hypopharyngeal gland in honey bees.
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影响因子：
16
作者：
Leung AK;Sharp PA
通讯作者：
Sharp PA
影响因子：
4.6
作者：
Collins DH;Mohorianu I;Beckers M;Moulton V;Dalmay T;Bourke AF
通讯作者：
Bourke AF
影响因子：
4.6
作者：
Freitas FC;Pires CV;Claudianos C;Cristino AS;Simões ZL
通讯作者：
Simões ZL
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
DOI：
10.1093/bioinformatics/btp692
发表时间：
2010-02-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Li B;Ruotti V;Stewart RM;Thomson JA;Dewey CN
通讯作者：
Dewey CN