Local targets of T-stellate cells in the ventral cochlear nucleus.
Local targets of T-stellate cells in the ventral cochlear nucleus.
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DOI：
10.1002/cne.25378
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发表时间：
2022-11
影响因子：
2.5
通讯作者：
Smith, Philip H.
中科院分区：
文献类型：
--
作者：
Lin, Lin;Campbell, Jay;Oertel, Donata;Smith, Philip H.
已知腹侧耳蜗核（VCN）中的T-星状细胞具有局部轴突侧支，其终止于与支配它们的听觉神经纤维平行的同一隔板内的树突和细胞体附近。假设在一氧化氮介导的上调T星状细胞对听觉输入的反应中，存在着同层内星状细胞之间的兴奋性突触连接。这可以作为一种机制的可变增益控制的元音频谱峰的响应增强。以前的研究为T星状细胞之间可能的突触互连提供了间接证据，但尚未建立明确的鉴定。在这里，我们使用逆行神经元追踪，将腺相关病毒载体或生物素化葡聚糖胺注射到下丘（IC）中，以检测VCN内T星状细胞的突触后靶点。我们发现，回填的T-星状细胞轴突上的标记的细胞体和树突上的其他标记的T-星状细胞在一个isopterylamma的单突触连接。电子显微镜显示，T-星状终末也可以在IC未逆行标记的结构上形成突触。甘氨酸抗体与病毒标记相结合表明，标记的T-星状终末突触的这些非回填结构最有可能是两种大小类别的甘氨酸能VCN细胞的细胞体和树突，其大小和相对数量表明它们是D-和L-星状细胞。已知这些细胞将抑制性输入提供回T星状细胞。我们的数据表明，除了他们的听觉神经输入，T-星状细胞提供了第二个可调节的兴奋性输入，抑制和兴奋性细胞在VCN iservellament，并可能在声学信息处理中发挥重要作用。除了它们的听觉神经输入，我们发现，T-星状细胞内的一个给定的issulamina提供第二个可调节的单突触兴奋性输入到其他T-星状细胞以及抑制L-和D-星状细胞。该电路在声学信息处理中可能发挥重要作用。
T‐stellate cells in the ventral cochlear nucleus (VCN) are known to have local axon collaterals that terminate in the vicinity of their dendrites and cell bodies within the same isofrequency lamina in parallel with the auditory nerve fibers that innervate them. Excitatory synaptic connections between stellate cells within an isofrequency lamina are hypothesized to be involved in the nitric oxide‐mediated upregulation of T‐stellate responses to their auditory input. This could serve as a mechanism of variable gain control in the enhancement of responses to vowel spectral peaks. Previous studies have provided indirect evidence for these possible synaptic interconnections between T‐stellate cells, but unequivocal identification has yet to be established. Here, we used retrograde neuronal tracing with adeno‐associated viral vector or biotinylated dextran amine injected into the inferior colliculus (IC) to detect the postsynaptic target of T‐stellate cells within the VCN. We show that backfilled T‐stellate cell axons make monosynapatic connections on the labeled cell bodies and dendrites of other labeled T‐stellate cells within an isofrequency lamina. Electron microscopy revealed that T‐stellate terminals can also make synapses on structures not retrogradely labeled from the IC. Glycine antibodies combined with the viral labeling indicated that these nonbackfilled structures that the labeled T‐stellate terminals were synapsing on are most likely the cell bodies and dendrites of two size categories of glycinergic VCN cells, whose sizes and relative numbers indicated they are the D‐ and L‐stellate cells. These cells are known to provide inhibitory inputs back onto T‐stellate cells. Our data indicate that, in addition to their auditory nerve input, T‐stellate cells provide a second modulatable excitatory input to both inhibitory and excitatory cells in a VCN isofrequency lamina and may play a significant role in acoustic information processing. In addition to their auditory nerve inputs, we show that T‐stellate cells within a given isofrequency lamina provide a second modulatable monosynaptic excitatory input to other T‐stellate cells as well as to inhibitory L‐ and D‐stellate cells. This circuit may play a significant role in acoustic information processing.
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DOI：
10.1111/ejn.12580
发表时间：
2014-07
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Coomber B;Berger JI;Kowalkowski VL;Shackleton TM;Palmer AR;Wallace MN
通讯作者：
Wallace MN
影响因子：
3.3
作者：
CANT, NB
通讯作者：
CANT, NB
DOI：
10.1007/s10162-008-0155-6
发表时间：
2009-06-01
影响因子：
2.4
作者：
Doucet, John R.;Lenihan, Nicole M.;May, Bradford J.
通讯作者：
May, Bradford J.
影响因子：
2.5
作者：
BLACKBURN, CC;SACHS, MB
通讯作者：
SACHS, MB
影响因子：
2.5
作者：
Gai, Yan;Carney, Laurel H.
通讯作者：
Carney, Laurel H.