Neural changes accompanying tinnitus following unilateral acoustic trauma in the guinea pig.
Neural changes accompanying tinnitus following unilateral acoustic trauma in the guinea pig.
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DOI：
10.1111/ejn.12580
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发表时间：
2014-07
期刊：
影响因子：
--
通讯作者：
Wallace MN
中科院分区：
文献类型：
--
作者：
Coomber B;Berger JI;Kowalkowski VL;Shackleton TM;Palmer AR;Wallace MN
耳鸣的动物模型使我们能够研究神经活动的变化和耳鸣感受之间的关系。在这里，豚鼠受到单侧噪声创伤，并在8周后进行耳鸣行为测试。通过比较耳鸣动物和没有耳鸣的动物，所有这些动物都暴露在噪音中，我们能够识别耳鸣组特有的变化。三个生理标志物已知的变化噪声暴露进行了检查：自发放电率（SFR）和突发放电下丘（IC），诱发听觉脑干反应（ABR），耳蜗核中含有一氧化氮合酶（NOS）的神经元的数量。我们在16只动物中的12只（75%）中获得了耳鸣的行为证据。所有暴露于噪声的动物的IC中的SFR和突发放电的发生率均升高，但耳鸣和无耳鸣动物之间没有差异。耳鸣动物同侧ABR潜伏期显著降低，与小听力损失的预期相反。此外，有一个同侧对侧的不对称性NOS染色的腹侧耳蜗核（VCN），这是唯一明显的耳鸣动物。耳鸣的动物有显着更多的含NOS神经元的噪音暴露侧，而没有耳鸣的动物没有。这些数据表明，测量NOS的VCN和记录ABR的补充行为的方法来确认动物耳鸣，一氧化氮参与与耳鸣相关的可塑性神经变化。
Animal models of tinnitus allow us to study the relationship between changes in neural activity and the tinnitus percept. Here, guinea pigs were subjected to unilateral noise trauma and tested behaviourally for tinnitus 8 weeks later. By comparing animals with tinnitus with those without, all of which were noise-exposed, we were able to identify changes unique to the tinnitus group. Three physiological markers known to change following noise exposure were examined: spontaneous firing rates (SFRs) and burst firing in the inferior colliculus (IC), evoked auditory brainstem responses (ABRs), and the number of neurons in the cochlear nucleus containing nitric oxide synthase (NOS). We obtained behavioural evidence of tinnitus in 12 of 16 (75%) animals. Both SFRs and incidences of burst firing were elevated in the IC of all noise-exposed animals, but there were no differences between tinnitus and no-tinnitus animals. There were significant decreases in ipsilateral ABR latencies in tinnitus animals, contrary to what might be expected with a small hearing loss. Furthermore, there was an ipsilateral–contralateral asymmetry in NOS staining in the ventral cochlear nucleus (VCN) that was only apparent in tinnitus animals. Tinnitus animals had a significantly greater number of NOS-containing neurons on the noise-exposed side, whereas no-tinnitus animals did not. These data suggest that measuring NOS in the VCN and recording ABRs supplement behavioural methods for confirming tinnitus in animals, and that nitric oxide is involved in plastic neural changes associated with tinnitus.
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影响因子：
56.9
作者：
BUCHWALD, JS;HUANG, CM
通讯作者：
HUANG, CM
DOI：
10.1007/s101620010030
发表时间：
2001-03-01
影响因子：
2.4
作者：
Bauer, CA;Brozoski, TJ
通讯作者：
Brozoski, TJ
影响因子：
2.5
作者：
DeBusk, BC;DeBruyn, EJ;Bonds, AB
通讯作者：
Bonds, AB
影响因子：
7.3
作者：
BRIGNOLA, G;CALIGNANO, A;DIROSA, M
通讯作者：
DIROSA, M
影响因子：
3.3
作者：
Coote, E. J.;Rees, A.
通讯作者：
Rees, A.