BAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH).
BAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH).
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BAC到未来！或寡核苷酸：微阵列比较基因组杂交（阵列CGH）的视角。
DOI：
10.1093/nar/gkj456
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发表时间：
2006
影响因子：
14.9
通讯作者：
Meijer, GA
中科院分区：
文献类型：
--
作者：
Ylstra, B;van den IJssel, P;Carvalho, B;Brakenhoff, RH;Meijer, GA
阵列CGH技术（阵列比较基因组杂交）已被开发用于在全基因组和/或高分辨率尺度上检测染色体拷贝数变化。它用于人类遗传学和肿瘤学，具有很大的临床应用前景。直到最近，主要PCR扩增的细菌人工染色体（BAC）或cDNA已被点样作为阵列上的元素。制造阵列所需的大规模DNA分离或大插入克隆的PCR扩增是复杂且耗时的。缺乏高分辨率高灵敏度（商业）替代品无疑阻碍了阵列CGH在研究和诊断中的实施。最近，作为阵列元件的合成寡核苷酸已经被学术机构以及商业提供商引入作为阵列CGH的替代基底。寡核苷酸库或现成的阵列可以现成购买，节省大量时间和精力。对于RNA表达谱分析，我们已经看到从内部打印的基于cDNA的表达阵列逐渐过渡到寡核苷酸阵列，我们预计阵列CGH也会出现类似的过渡。本文比较了不同的平台，并将试图照亮阵列计算全息技术的“BAC的未来”。
The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is used in human genetics and oncology, with great promise for clinical application. Until recently primarily PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted as elements on the array. The large-scale DNA isolations or PCR amplifications of the large-insert clones necessary for manufacturing the arrays are elaborate and time-consuming. Lack of a high-resolution highly sensitive (commercial) alternative has undoubtedly hindered the implementation of array CGH in research and diagnostics. Recently, synthetic oligonucleotides as arrayed elements have been introduced as an alternative substrate for array CGH, both by academic institutions as well as by commercial providers. Oligonucleotide libraries or ready-made arrays can be bought off-the-shelf saving considerable time and efforts. For RNA expression profiling, we have seen a gradual transition from in-house printed cDNA-based expression arrays to oligonucleotide arrays and we expect a similar transition for array CGH. This review compares the different platforms and will attempt to shine a light on the ‘BAC to the future’ of the array CGH technique.
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影响因子：
14.9
作者：
Egeland RD;Southern EM
通讯作者：
Southern EM
影响因子：
20.3
作者：
Schreurs, MWJ;Hermsen, MAJA;Hooijberg, E
通讯作者：
Hooijberg, E
影响因子：
56.9
作者：
Sebat, J;Lakshmi, B;Wigler, M
通讯作者：
Wigler, M
子宫颈癌中染色体失衡的微阵列比较基因组杂交检测。
影响因子：
3.8
作者：
Hidalgo, A;Baudis, M;Petersen, I;Arreola, H;Piña, P;Vázquez-Ortiz, G;Hernández, D;González, J;Lazos, M;López, R;Pérez, C;García, J;Vázquez, K;Alatorre, B;Salcedo, M
通讯作者：
Salcedo, M
影响因子：
4.1
作者：
DeVries, S;Nyante, S;Waldman, F
通讯作者：
Waldman, F