Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication.
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication.
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DOI：
10.1093/nar/gni117
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发表时间：
2005-08-05
影响因子：
14.9
通讯作者：
Southern EM
中科院分区：
文献类型：
--
作者：
Egeland RD;Southern EM
我们展示了一种新的方法，使寡核苷酸微阵列原位合成。该方法使用常规的DNA合成化学与电化学去封闭步骤。酸被递送到载玻片上的特定区域，从而仅允许在选定的位点添加核苷酸。酸是通过由独立的微电极阵列控制的电化学氧化产生的。当竞争性副产物反应最小时，解封闭在几秒钟内完成。我们证明了17聚体的成功合成和单碱基对错配杂交的歧视。本研究中生成的特征宽度为40 μm，边缘清晰。该合成技术可应用于其他分子阵列的制备。
We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 μm wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays.
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影响因子：
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作者：
MEYER, H;DREWER, H;ROSPERT, M
通讯作者：
ROSPERT, M
影响因子：
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Okamoto, T;Suzuki, T;Yamamoto, N
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Yamamoto, N
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Singh-Gasson, S;Green, RD;Cerrina, F
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Cerrina, F
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作者：
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Septak, M
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作者：
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Linsley, PS