Erchen Plus Huiyanzhuyu Decoction Inhibits the Growth of Laryngeal Carcinoma in a Mouse Model of Phlegm-Coagulation-Blood-Stasis Syndrome via the STAT3/Cyclin D1 Pathway
Erchen Plus Huiyanzhuyu Decoction Inhibits the Growth of Laryngeal Carcinoma in a Mouse Model of Phlegm-Coagulation-Blood-Stasis Syndrome via the STAT3/Cyclin D1 Pathway
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二陈加会咽逐瘀汤通过STAT3/Cyclin D1通路抑制痰凝血瘀证小鼠喉癌生长
DOI：
10.1155/2020/2803496
复制
发表时间：
2020-04
影响因子：
--
通讯作者：
Li Yunying
中科院分区：
文献类型：
--
作者：
Tan Xi;Luo Qiulan;Zhou Shiqing;Huang Wei;Feng Xiaocong;Chen Wenyong;Yang Chaojie;Li Yunying
Erchen plus Huiyanzhuyu decoction (EHD), a Chinese herbal medicine (CHM) formula that consists of Erchen decoction and Huiyanzhuyu decoction, has achieved satisfactory results in the clinic. The main function of EHD is to remove phlegm and blood stasis, and EHD is suitable for phlegm-coagulation-blood-stasis (PCBS) syndrome in laryngeal cancer (LC). In this study, a xenograft mouse model of LC with PCBS syndrome was constructed by feeding a high-fat diet, swimming in ice water, and subcutaneously injecting epinephrine hydrochloride for 2 weeks. Based on the successful Chinese medicine syndrome model, Hep2-luciferase-GFP cells were injected subcutaneously under the armpit of the right upper limb in mice to form tumours. A mouse model of LC with PCBS syndrome was established via heterotopic transplantation. Then, the mice received intragastric administration of different concentrations of EHD daily, and cisplatin (DDP) was intraperitoneally injected every week for 21 days. Tumour fluorescence in mice was measured with a living animal imager on days 7, 14, 21, and 28 during treatment. The results of this experiment confirmed that a mouse model of Chinese medicine syndrome was successfully constructed. Moreover, EHD slowed the growth of xenograft tumours in nude mice; decreased the expression levels of STAT3, p-STAT3, and cyclin D1; and upregulated the expression level of P27. In brief, EHD inhibited laryngeal tumour growth in a xenograft mouse model of PCBS syndrome and regulated the STAT3/cyclin D1 signalling pathway. This study was the first to construct a Chinese medicine xenograft mouse model of LC with PCBS syndrome; in addition, this study clarified that EHD regulated the STAT3/cyclin D1 signalling pathway to inhibit the growth of LC and that EHD may be a promising novel therapeutic compound for the treatment of patients with LC.
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DOI：
10.1155/2017/5350161
发表时间：
2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Sen Z;Jie M;Jingzhi Y;Dongjie W;Dongming Z;Xiaoguang C
通讯作者：
Xiaoguang C
DOI：
--
发表时间：
2018
期刊：
--
影响因子：
--
作者：
Ying Yang;Juyi Wen;Jingjiao Li;Yuanyuan Zhang;Henghu Fang;Sudong Wu;Tingjun Dong;Panpan;Gan;C. Cai
通讯作者：
Ying Yang;Juyi Wen;Jingjiao Li;Yuanyuan Zhang;Henghu Fang;Sudong Wu;Tingjun Dong;Panpan;Gan;C. Cai
影响因子：
11.2
作者：
Leslie, K;Lang, C;Bromberg, J
通讯作者：
Bromberg, J
影响因子：
7.5
作者：
Yu, Shuo;Gong, Lian-sheng;Zhang, Lun
通讯作者：
Zhang, Lun
影响因子：
8.2
作者：
Gao, LF;Xu, DQ;Zhao, XJ
通讯作者：
Zhao, XJ