The Importance of Mimicking Dermal-Epidermal Junction for Skin Tissue Engineering: A Review.
The Importance of Mimicking Dermal-Epidermal Junction for Skin Tissue Engineering: A Review.
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DOI：
10.3390/bioengineering8110148
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发表时间：
2021-10-20
期刊：
影响因子：
--
通讯作者：
Claeyssens F
中科院分区：
文献类型：
--
作者：
Aleemardani M;Trikić MZ;Green NH;Claeyssens F
人体皮肤的真皮和表皮之间有一个明显的边界，称为基底膜，这是一个致密的胶原蛋白网络，形成真皮-表皮连接处 (DEJ) 的起伏。 DEJ 在皮肤稳态和功能中发挥多种作用，即增强各层的粘附和物理连锁、为表皮干细胞创造生态位、调节细胞微环境以及在成纤维细胞和角质形成细胞之间提供物理边界层。然而，DEJ 的主要作用已被确定为皮肤完整性；其中仍有一些方面尚未得到充分调查。组织工程 (TE) 已经发展出有前景的皮肤再生策略，并且已经开发出可供临床使用的 TE 支架。然而，目前可用的皮肤 TE 等效物忽略了复制 DEJ 解剖结构。生产日益复杂的皮肤 TE 支架的新兴能力将使开发更接近天然皮肤的物理和生理模拟成为可能；它还允许研究人员研究 DEJ 对细胞功能的影响。很少有研究创造出可以模仿人类 DEJ 的图案化基底来探索其意义。在这里，我们首先回顾 DEJ 角色，然后批判性地讨论创建 DEJ 波动结构的 TE 策略及其效果。该领域的新方法可能有助于改进生物工程皮肤替代品、创造 3D 工程皮肤、识别病理机制以及生产和筛选药物。
There is a distinct boundary between the dermis and epidermis in the human skin called the basement membrane, a dense collagen network that creates undulations of the dermal–epidermal junction (DEJ). The DEJ plays multiple roles in skin homeostasis and function, namely, enhancing the adhesion and physical interlock of the layers, creating niches for epidermal stem cells, regulating the cellular microenvironment, and providing a physical boundary layer between fibroblasts and keratinocytes. However, the primary role of the DEJ has been determined as skin integrity; there are still aspects of it that are poorly investigated. Tissue engineering (TE) has evolved promising skin regeneration strategies and already developed TE scaffolds for clinical use. However, the currently available skin TE equivalents neglect to replicate the DEJ anatomical structures. The emergent ability to produce increasingly complex scaffolds for skin TE will enable the development of closer physical and physiological mimics to natural skin; it also allows researchers to study the DEJ effect on cell function. Few studies have created patterned substrates that could mimic the human DEJ to explore their significance. Here, we first review the DEJ roles and then critically discuss the TE strategies to create the DEJ undulating structure and their effects. New approaches in this field could be instrumental for improving bioengineered skin substitutes, creating 3D engineered skin, identifying pathological mechanisms, and producing and screening drugs.
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DOI：
10.1111/j.1468-3083.2007.02326.x
发表时间：
2008-01-01
影响因子：
9.2
作者：
Bannasch, H.;Stark, G. B.;Foehn, M.
通讯作者：
Foehn, M.
DOI：
10.1038/nrm3591
发表时间：
2013-06
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.1
作者：
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通讯作者：
Pins, George D.
影响因子：
3.4
作者：
Aleemardani, Mina;Solouk, Atefeh;Moeini, Mohammad
通讯作者：
Moeini, Mohammad
影响因子：
4
作者：
Estrach, Soline;Legg, James;Watt, Fiona M.
通讯作者：
Watt, Fiona M.