Inhibition of HIV-1 replication by RNA with a microRNA-like function.
Inhibition of HIV-1 replication by RNA with a microRNA-like function.
复制标题
具有类似 microRNA 功能的 RNA 抑制 HIV-1 复制。
DOI：
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发表时间：
2013
影响因子：
5.4
通讯作者：
H. Takaku
中科院分区：
文献类型：
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作者：
Keijiro Kato;Takashi Senoki;H. Takaku
人类免疫缺陷病毒1型（HIV-1）的复制受到抑制， 靶向HIV-1 RNA包装信号的小向导RNA（sgRNA）。我们无意中 产生了具有sgRNA的反向序列及其终止子的质粒（pR-SgRNA-ter）。 sgRNA和R-sgRNA都能抑制HIV-1，但R-sgRNA抑制HIV-1的机制还不清楚。 抑制艾滋病病毒还不清楚。评估抑制效应是否由 通过RNA干扰或microRNA（miRNA）样机制， 在体外用Dicer酶处理，Dicer酶是RNA干扰的重要酶 和miRNA。RNA被切割成大约24个核苷酸的片段 （nt）。我们分析了RNA片段的序列，并预测了RNA二级结构。 R-RNA-sgRNA的结构以确定Dicer酶识别的区域。 R-SgRNA片段的长度范围为48 - 140 nt， 在这个区域形成双链，包括错配。HIV-1 p24检测 表明R-SgRNA片段抑制HIV-1复制。这些发现 提示R-RNA-sgRNA作为miRNA抑制HIV-1。
Human immunodeficiency virus type 1 (HIV-1) replication is suppressed by a small guide RNA (sgRNA) that targets the packaging signal of HIV-1 RNA. We unintentionally produced a plasmid with the reverse sequence of the sgRNA and its terminator (pR-Ψ-sgRNA-ter). Both sgRNA and R-Ψ-sgRNA suppress HIV-1, but the mechanism by which R-Ψ-sgRNA suppresses HIV is not clear. To evaluate whether the suppressive effect is caused by an RNA interference or microRNA (miRNA)-like mechanism, R-Ψ-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important enzyme for RNA interference and miRNA. The RNA was cleaved into fragments of approximately 24 nucleotides (nt). We analyzed the sequence of the RNA fragments and predicted the RNA secondary structure of R-Ψ-sgRNA to determine the region recognized by the Dicer enzyme. The lengths of the R-Ψ-sgRNA fragments ranged from 48 to 140 nt, and were predicted to form double strands, including mismatches, in this region. An HIV-1 p24 assay indicated that the R-Ψ-sgRNA fragments suppressed HIV-1 replication. These findings suggest that R-Ψ-sgRNA acts as a miRNA to inhibit HIV-1.
影响因子：
10.5
作者：
Grivna, Shane T.;Beyret, Ergin;Lin, Haifan
通讯作者：
Lin, Haifan