Studies on the cyclic AMP response to thyroid stimulating immunoglobulin (TSI) and thyrotropin (TSH) in human thyroid cell monolayers.
Studies on the cyclic AMP response to thyroid stimulating immunoglobulin (TSI) and thyrotropin (TSH) in human thyroid cell monolayers.
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研究人甲状腺细胞单层中环 AMP 对促甲状腺免疫球蛋白 (TSI) 和促甲状腺素 (TSH) 的反应。
DOI：
10.1016/0026-0495(82)90168-8
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Halverson,G
中科院分区：
文献类型：
--
作者：
Rapoport,B;Filetti,S;Takai,N;Seto,P;Halverson,G
对培养的人甲状腺细胞进行了促甲状腺免疫球蛋白(TSI)和促甲状腺激素(TSH)的生物测定。为了证实Kasagi等人的数据，人的甲状腺细胞在缺乏氯化钠的Hank平衡盐溶液中孵育后，c-AMP对牛TSH的敏感性增加了大约一个数量级。TSI+GT、HTSH+GT、bTSH对培养液中氯化钠的刺激效果最好。与在NaC l(+)介质中孵育相比，在NaC l(−)介质中，产生的c-AMP的大部分(70%-80%)被释放到介质中；这一比例在较大的bTSH和HTSH浓度范围内保持相对恒定。当TSI浓度高于3 mg/ml时，c-AMP外流到培养液中的量明显减少。在NaC l(−)中，霍乱毒素和前列腺素E对甲状腺细胞c-AMP生成的刺激作用与TSH和TSI的刺激作用相反。在NaC l(+)介质中加入10−4M放线菌酮可增强c-AMP对生理浓度促甲状腺激素的反应。与NaC l(−)介质一样，放线菌酮增加了c-AMP对促甲状腺激素反应的敏感性，但不是最大反应。但在NaC l(−)介质和放线菌亚胺中没有观察到加和性。从Graves病患者体内获得的人甲状腺细胞对TSI刺激相对不敏感。然而，在NaC l(−)介质中，这些细胞对TSI刺激的敏感性足以使它们用于TSI检测。对TSI分析的现状进行了讨论。
Studies were conducted on the cultured human thyroid cell bioassay for thyroid stimulating immunoglobulin (TSI) and thyrotropin (TSH). In confirmation of the data of Kasagi, et al.,15incubation of human thyroid cells in Hank's balanced salt solution deficient in NaCl increased the sensitivity of the c-AMP response to bovine TSH by approximately one order of magnitude. Half-maximal stimulation was attained at approximately 0.1 mU bTSH/ml. The effect of NaCl in the medium was greatest with stimulation by TSI > hTSH > bTSH. In contrast to incubations in NaCl(+) medium, with NaCl(−) medium most (70%–80%) of the c-AMP produced was released into the medium; this proportion remaining relatively constant over a wide range of bTSH and hTSH concentrations. At TSI concentrations higher than 3mg/ml efflux of c-AMP into the medium was significantly diminished. Stimulation by cholera toxin and prostaglandin E of thyroid cell c-AMP generation was not enhanced in NaCl(−) medium, in contrast to stimulation by TSH and TSI. The presence of 10−4M cycloheximide in NaCl(+) medium enhanced the c-AMP response to physiological concentrations of TSH. As with NaCl(−) medium, cycloheximide increased the sensitivity but not the maximum response of the c-AMP response to TSH. However no additivity was observed with NaCl(−) medium and cycloheximide. Human thyroid cells obtained from patients with Graves' disease are relatively insensitive to TSI stimulation. In NaCl(−) medium, however, the sensitivity of these cells to TSI stimulation is sufficient to enable them to be utilized in the TSI assay. The present state of the TSI assay is discussed.
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DOI：
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发表时间：
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期刊：
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