Corticosterone regulates the expression of neuropeptide Y and reelin in MLO-Y4 cells
Corticosterone regulates the expression of neuropeptide Y and reelin in MLO-Y4 cells
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皮质酮调节 MLO-Y4 细胞中神经肽 Y 和 reelin 的表达
DOI：
10.1007/s10059-012-0053-y
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发表时间：
2012-05
影响因子：
3.8
通讯作者：
Wang, Hang
中科院分区：
文献类型：
--
作者：
Fu, Jing;Shen, Jiefei;Li, Xiaoyu;Wang, Hang
人们普遍认为，具有树突状外观的骨细胞形成了复杂的细胞网络系统，并作为主要的骨机械传感器在机械转导中发挥着至关重要的作用，这是其神经元样生物学的基础，如先前报道的。神经肽 Y (NPY) 和 reelin mRNA 是大脑特异性神经源性标记物，已在骨细胞中被发现。然而，NPY 和 reelin 的产生因特定生化刺激而发生的变化尚不清楚。在本研究中，我们研究了内源性糖皮质激素之一皮质酮对 MLO-Y4 骨细胞系中 NPY 和 reelin 表达的体外影响。用不同浓度（10−9M-10−5M）的皮质酮处理细胞1、3、6、12和24小时。根据 MTT 测定和 Vi-CELL 分析仪显示，皮质酮以剂量和时间依赖性方式降低 MLO-Y4 细胞活力和增殖。然后将细胞与皮质酮 (10−6μM) 一起孵育，并使用实时 PCR 和蛋白质印迹分析在 1、3、6、12 和 24 小时检测 NPY 和 reelin 表达水平。这些结果表明，在基因和蛋白质水平上，皮质酮以时间依赖性方式显着上调 NPY 和 reelin 表达。糖皮质激素受体拮抗剂RU486的应用逆转了皮质酮引起的细胞活力降低以及NPY和reelin表达增加。据我们所知，这是第一份证实皮质酮调节骨细胞中 NPY 和 reelin 表达的报告。
Osteocytes that have a dendritic appearance are widely believed to form a complex cellular network system and play crucial roles in mechanotransduction as a principal bone mechanosensor, which is the basis of their neuronallike biology, as previously reported. Neuropeptide Y (NPY) and reelin mRNA, which are brain-specific neurogenic markers, have been identified in osteocytes. However, changes in the production of NPY and reelin in response to specific biochemical stimulation are unknown. In this study, we investigated thein vitroeffect of corticosterone, one of the endogenous glucocorticoids, on the expression of NPY and reelin in the MLO-Y4 osteocyte cell line. Cells were treated with corticosterone at different concentrations (10−9M-10−5M) for 1, 3, 6, 12 and 24 h. As revealed, corticosterone reduced the MLO-Y4 cell viability and proliferation in a dose- and time-dependent manner based on an MTT assay and a Vi-CELL analyzer. The cells were then incubated with corticosterone (10−6μM), and the NPY and reelin expression levels were detected at 1, 3, 6, 12 and 24 h using real-time PCR and Western blot analysis. These results demonstrated that at the gene and the protein levels, corticosterone significantly upregulated the NPY and reelin expression in a time-dependent manner. The application of a glucocorticoid receptor antagonist, RU486, reversed the reduced cell viability and the increased expression of NPY and reelin that were caused by corticosterone. To the best of our knowledge, this is the first report to verify that corticosterone regulates the NPY and reelin expression in osteocytes.
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影响因子：
25.7
作者：
Kobold, D;Grundmann, A;Knittel, T
通讯作者：
Knittel, T
影响因子：
3.5
作者：
Satoko Suga;S. Goto;A. Togari
通讯作者：
Satoko Suga;S. Goto;A. Togari
影响因子：
4
作者：
Igwe, John C.;Jiag, Xi;Paic, France;Ma, Li;Adams, Douglas J.;Baldock, Paul A.;Pilbeam, Carol C.;Kalajzic, Ivo
通讯作者：
Kalajzic, Ivo
影响因子：
4.4
作者：
Royaux, I;deRouvroit, CL;Goffinet, AM
通讯作者：
Goffinet, AM
影响因子：
4
作者：
Liliana Teixeira;D. Sousa;A. Nunes;M. Sousa;H. Herzog;M. Lamghari
通讯作者：
Liliana Teixeira;D. Sousa;A. Nunes;M. Sousa;H. Herzog;M. Lamghari