Desiccation tolerance in the chlorophyte green alga Ulva compressa: does cell wall architecture contribute to ecological success?
Desiccation tolerance in the chlorophyte green alga Ulva compressa: does cell wall architecture contribute to ecological success?
复制标题
DOI：
10.1007/s00425-015-2292-6
复制
发表时间：
2015-08
期刊：
影响因子：
4.3
通讯作者：
Lewis LA
中科院分区：
文献类型：
--
作者：
Holzinger A;Herburger K;Kaplan F;Lewis LA
干燥导致扁穗石榴内果胶细胞壁层结构的变化。这有助于防止干燥-再水化循环造成的机械损伤。扁石榴属是地中海常见的一种，具有rbcL系统发育特征。石杉作为潮间带物种，在自然界中耐受反复的干燥-复水循环，在实验条件下对其生理和结构基础进行了研究。干燥到73%的相对含水量(RWC)导致光系统II的最大量子效率(Fv/Fm)显着下降到初始值的一半左右。减少到48%或27%的RWC会造成更剧烈的影响，而Talli只能从干燥完全恢复到73%的RWC。相对电子传递速率在73%RWC时被刺激，但在48%和27%RWC时显著降低。成像-PAM分析显示，单个菌体盘内均一干燥过程。用标准的染色方法对不同的细胞壁层进行了表征，即结构成分(纤维素类、胼胝质)为钙荧光白色和苯胺蓝，果胶为Ru红，酸性多糖为甲苯胺蓝。RWC已降至73%，导致细胞壁发生严重变化。内部富含果胶的层遵循细胞质的收缩过程，而外部致密的纤维层保持其形状。通过这种方式，菌体在失水期间不会发生质壁分解，在恢复时也不会受到任何机械损伤的负面影响。透射电子显微镜证实了不同层的排列，通过它们的质地和电子密度可以清楚地区分出来。我们认为富含果胶的细胞壁层的灵活性是Ulva对干燥耐性的主要贡献。本文的在线版本(doi：10.1007/s00425-0152292-6)包含补充材料，授权用户可以使用。
Desiccation leads to structural changes of the inner pectic cell wall layers inUlva compressa. This contributes to protection against mechanical damage due to desiccation–rehydration cycles. Ulva compressa, characterized by rbcL phylogeny, is a common species in the Mediterranean Sea. Ulva as an intertidal species tolerates repeated desiccation–rehydration cycles in nature; the physiological and structural basis were investigated under experimental conditions here. Desiccation to 73 % relative water content (RWC) led to a significant decrease of the maximum quantum yield of photosystem II (Fv/Fm) to about half of the initial value. A reduction to 48 or 27 % RWC caused a more drastic effect and thalli were only able to recover fully from desiccation to 73 % RWC. Relative electron transport rates were stimulated at 73 % RWC, but decreased significantly at 48 and 27 % RWC, respectively. Imaging-PAM analysis demonstrated a homogenous desiccation process within individual thallus discs. The different cell wall layers of U. compressa were characterized by standard staining procedures, i.e. calcofluor white and aniline blue for structural components (cellulose, callose), ruthenium red for pectins and toluidine blue for acidic polysaccharides. Already a reduction to 73 % RWC caused severe changes of the cell walls. The inner pectin-rich layers followed the shrinkage process of the cytoplasm, while the outer denser fibrillar layers maintained their shape. In this way, the thalli were not plasmolyzed during water loss, and upon recovery not negatively influenced by any mechanical damage. Transmission electron microscopy corroborated the arrangement of the different layers clearly distinguishable by their texture and electron density. We suggest the flexibility of the pectin-rich cell wall layers as a major contribution to desiccation tolerance in Ulva. The online version of this article (doi:10.1007/s00425-015-2292-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
绿潮主要致病物种——石莼的增殖策略，通过破碎形成孢子囊
影响因子：
3.7
作者：
Gao S;Chen X;Yi Q;Wang G;Pan G;Lin A;Peng G
通讯作者：
Peng G
影响因子：
5.6
作者：
Domozych DS;Ciancia M;Fangel JU;Mikkelsen MD;Ulvskov P;Willats WG
通讯作者：
Willats WG
影响因子：
6.9
作者：
Bischof, K;Peralta, G;Breeman, AM
通讯作者：
Breeman, AM
影响因子：
3
作者：
Contreras-Porcia, Loretto;Dennett, Geraldine;Moenne, Alejandra
通讯作者：
Moenne, Alejandra
影响因子：
4.3
作者：
DICKINSON, DMJ;JONES, RGW;DAVENPORT, J
通讯作者：
DAVENPORT, J