Patrinoside and Patrinoside A from Patrinia scabiosaefolia Improve Insulin Resistance by Inhibiting NF-κB, MAPK Pathways and Oxidative Stress in RAW264.7 and 3 T3-L1 Cells.
Patrinoside and Patrinoside A from Patrinia scabiosaefolia Improve Insulin Resistance by Inhibiting NF-κB, MAPK Pathways and Oxidative Stress in RAW264.7 and 3 T3-L1 Cells.
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来自败酱草的败酱苷和败酱苷 A 通过抑制 RAW264.7 和 3-T3-L1 细胞中的 NF-κB、MAPK 通路和氧化应激来改善胰岛素抵抗
DOI：
10.1155/2023/9069645
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发表时间：
2023
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
败酱草作为传统的药食两用植物，在中国已有数千年的历史，用于治疗阑尾炎、肠炎、肝炎等。在我们之前的研究中，从 P. scabiosaefolia 中分离出的败酱苷和败酱苷 A 可以通过激活 PI-3 K/AKT 信号通路显着改善胰岛素抵抗（IR）。由于IR与炎症密切相关，因此评估它们在LPS诱导的RAW264.7炎症模型和TNF-α诱导的3 T3-L1 IR炎症模型中的抗炎活性，以确定改善IR的效果是否与抗炎活性相关。在RAW264.7细胞中，败酱苷和败酱苷A显着抑制炎症介质NO、TNF-α和IL-6的转录和分泌。 Western blot分析显示，对IκB、P65、P38、ERK、JNK磷酸化有显着抑制作用，提示该作用是通过NF-κB通路和MAPK通路发挥作用的。在3 T3-L1细胞中，败酱苷和败酱苷A还通过抑制炎症细胞因子IL-6以及趋化因子MCP-1和MIP-1α的转录来抑制NF-κB和MAPK通路的激活。这些事件导致巨噬细胞向脂肪细胞迁移的抑制。此外，败酱苷和败酱苷 A 通过抑制 LPS 刺激的 RAW264.7 细胞中 ROS 的释放来改善氧化应激。综上所述，败酱苷和败酱苷A可激活PI-3 K/AKT通路，抑制NF-κB通路、MAPK通路，改善氧化应激，具有改善IR的多途径作用。该研究结果为提高油菜IR的物质基础和机制提供了科学依据。
Patrinia scabiosaefolia, as traditional food and medicine plant, was used to treat appendicitis, enteritis, and hepatitis for thousand years in China. Patrinoside and patrinoside A isolated from P. scabiosaefolia could significantly improve insulin resistance (IR) by activating PI-3 K/AKT signaling pathway in our previous study. Since IR is closely related to inflammation, their anti-inflammatory activities in RAW264.7 inflammatory model induced by LPS and in 3 T3-L1 IR inflammatory model induced by TNF-α were evaluated to identify whether the effects on improving IR related to anti-inflammatory activity. In RAW264.7 cells, patrinoside and patrinoside A significantly inhibited the transcription and secretion of inflammatory mediators NO, TNF-α, and IL-6. Western blot analysis showed that the significant inhibition of phosphorylation of IκB and P65 and P38, ERK and JNK suggested that the effects were exerted through NF-κB pathway and MAPK pathway. In 3 T3-L1 cells, patrinoside and patrinoside A also inhibited the activation of NF-κB and MAPK pathways through inhibiting the transcriptions of inflammatory cytokines IL-6 and chemokines MCP-1 and MIP-1α. These events resulted in the inhibition of macrophages migration to adipocytes. In addition, patrinoside and patrinoside A ameliorated oxidative stress by inhibiting ROS release in LPS-stimulated RAW264.7 cells. In conclusion, patrinoside and patrinoside A could active PI-3 K/AKT pathway, inhibit NF-κB pathway, MAPK pathway, and improve oxidative stress, which showed multipathways on improving IR. These results provided the scientific basis for material basis and mechanism on improving IR of P. scabiosaefolia.
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