Increasing the stability of Lumbricus terrestris erythrocruorin via poly(acrylic acid) conjugation.
Increasing the stability of Lumbricus terrestris erythrocruorin via poly(acrylic acid) conjugation.
复制标题
通过聚（丙烯酸）结合来提高lumbricus terrestris rethrothocruorin的稳定性。
DOI：
10.1080/21691401.2018.1480491
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Elmer J
中科院分区：
文献类型：
--
作者：
Spivack K;Tucker M;Zimmerman D;Nicholas M;Abdulmalik O;Comolli N;Elmer J
由于捐献的红细胞必须不断冷藏，因此在偏远地区和战场上往往无法获得。这项研究的目标是合成一种不需要冷藏的高度稳定的血液替代品。具体地说，细胞外血红蛋白(又称以聚丙烯酸(PAA)和乙二胺(EDA)为交联剂，对蚯蚓的红细胞素(EC)进行了研究。PAGE分析表明，亚基之间存在交联性，而动态光散射和扫描电子显微镜显示，交联性显著增加了微球的尺寸(164±13.9 nm)。交联剂还显著提高了Ltd.Ec纳米颗粒的热稳定性，与天然的Ltd Ec(TM=62±0.6℃)相比，其热稳定性提高了10℃(TM=72±0.84℃)。此外，在pH为9的条件下，天然的LtEc迅速解离，但在pH为10的条件下，该纳米粒子能抵抗亚基的解离。其氧亲和力(P50=6.85±0.13 mm Hg)远高于天然的Ltd Ec(P50=26.67±0.4 mm Hg)，但其协同作用(n=2.43±0.12)不受影响。综上所述，这些结果表明，与PAA和EDA交联物提供了一种潜在的血液替代品，具有更高的稳定性和氧气亲和力。
Since donated red blood cells must be constantly refrigerated, they are often unavailable in remote areas and battlefields. The goal of this study was to synthesize a highly stable blood substitute that does not require refrigeration. Specifically, the extracellular hemoglobin (a.k.a. erythrocruorin, Ec) of the earthworm Lumbricus terrestris (LtEc) was cross-linked with poly(acrylic) acid (PAA) and ethylene diamine (EDA). PAGE analysis of the LtEc nanoparticles reveals cross-linking between subunits, while dynamic light scattering and scanning electron microscopy show that cross-linking significantly increases the size of the LtEc nanoparticles (164 ± 13.9 nm). Cross-linking also significantly increased the thermal stability of the LtEc nanoparticles by 10°C (Tm = 72 ± 0.84°C) relative to native LtEc (Tm = 62 ± 0.6°C). In addition, while native LtEc rapidly dissociates at pH 9, the LtEc nanoparticles resist subunit dissociation up to pH 10. The oxygen affinity of the LtEc nanoparticles (P50 = 6.85 ± 0.13 mmHg) is much higher than native LtEc (P50 = 26.67 ± 0.4 mmHg), but the cooperativity (n = 2.43 ± 0.12) is not affected. Altogether, these results show that cross-linking LtEc with PAA and EDA provides a potential blood substitute with increased stability and oxygen affinity.
登录
查看更多内容
影响因子：
3.7
作者：
Jani, Vivek P.;Jelvani, Alborz;Cabrales, Pedro
通讯作者：
Cabrales, Pedro
DOI：
10.1080/10731190600974376
发表时间：
2007-01-01
期刊：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
影响因子：
--
作者：
Li, Dongxia;Manjula, Belur N.;Acharya, A. Seetharama
通讯作者：
Acharya, A. Seetharama
DOI：
10.1016/0305-0491(81)90098-5
发表时间：
1981-01-01
影响因子：
2.2
作者：
OCHIAI, T;ENOKI, Y
通讯作者：
ENOKI, Y
影响因子：
3.1
作者：
Zapletal, Christina;Bode, Alexander;Golling, Markus
通讯作者：
Golling, Markus
DOI：
10.1016/j.ejpb.2009.03.008
发表时间：
2009-08-01
影响因子：
4.9
作者：
Greindl, Melanie;Foeger, Florian;Bernkop-Schnuerch, Andreas
通讯作者：
Bernkop-Schnuerch, Andreas