Design and Fabrication of a Novel Transplant Combined with Human Bone Marrow Mesenchymal Stem Cells and Platelet-rich Fibrin: New Horizons for Periodontal Tissue Regeneration after Dental Trauma
Design and Fabrication of a Novel Transplant Combined with Human Bone Marrow Mesenchymal Stem Cells and Platelet-rich Fibrin: New Horizons for Periodontal Tissue Regeneration after Dental Trauma
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结合人骨髓间充质干细胞和富含血小板的纤维蛋白的新型移植物的设计和制造：牙外伤后牙周组织再生的新视野
DOI：
10.22037/ijpr.2017.2136
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
M. Ayatollahi
中科院分区：
文献类型：
--
作者：
H. Moradian;A. Rafiee;M. Ayatollahi
拔除的牙齿干燥再植更容易丢失。最近的组织工程研究集中在制造各种细胞递送系统，以改善此类牙齿的整体预后。为了评估这种新的细胞移植方法，我们最初的目的是设计PRF支架，并确定BMMSCs在制造的支架上的活力和功能。为了在体外测试这一概念，分离人BMMSCs，并通过细胞表面标志物对其进行表征，以及其成骨/成脂分化的潜力。采用细胞增殖实验观察PRF支架对人骨髓间充质干细胞的生物学效应。对数据进行定量统计分析。结果发现，由于其生长因子的分泌，PRF在整个孵育期间显著诱导BMMSCs增殖。MTT法结果显示，在第1天至第7天的测试期间，MTT法结果呈增加趋势。扫描电镜结果显示，BMMSCs在接种后不久即能与纤维蛋白支架紧密粘附。这些数据表明，骨髓间充质干细胞/PRF构建体有可能改善临床预后再植撕脱牙在未来。在临床使用之前，需要进行更多的研究。
Avulsed teeth that are replanted dried are more prone to loss. Recent tissue engineering studies focus on fabrication of various cell delivery systems to improve the overall prognosis of such teeth. To evaluate this new cell transplant method, we initially aimed at designing of PRF scaffold and determining BMMSCs viability and function on the fabricated scaffold. To test this concept in-vitro, human BMMSCs were isolated and characterized by cell surface marker, and their potential on osteogenic/adipogenic differentiation. The biological effects of PRF scaffold on human BMMSCs were then investigated by cell proliferation assay. The data were quantified for statistical analysis. It was found that PRF significantly induced BMMSCs proliferation throughout the incubation period due to its growth factor secretion. Results of MTT assay showed an increasing trend during the testing period from day 1 to day 7. The result of scanning electron microscopy showed that BMMSCs could tightly adhere to fibrin scaffold just shortly after seeding. These data suggest that the BMMSCs/PRF construct has the potential to improve the clinical prognosis of replanted avulsed teeth in future. Additional studies are needed to be performed before its clinical use.
影响因子：
4.3
作者：
Kawaguchi, H;Hirachi, A;Kurihara, H
通讯作者：
Kurihara, H