Roles of dynein and dynactin in early endosome dynamics revealed using automated tracking and global analysis.
Roles of dynein and dynactin in early endosome dynamics revealed using automated tracking and global analysis.
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DOI：
10.1371/journal.pone.0024479
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Allan VJ
中科院分区：
文献类型：
--
作者：
Flores-Rodriguez N;Rogers SS;Kenwright DA;Waigh TA;Woodman PG;Allan VJ
Microtubule-dependent movement is crucial for the spatial organization of endosomes in most eukaryotes, but as yet there has been no systematic analysis of how a particular microtubule motor contributes to early endosome dynamics. Here we tracked early endosomes labeled with GFP-Rab5 on the nanometer scale, and combined this with global, first passage probability (FPP) analysis to provide an unbiased description of how the minus-end microtubule motor, cytoplasmic dynein, supports endosome motility. Dynein contributes to short-range endosome movement, but in particular drives 85–98% of long, inward translocations. For these, it requires an intact dynactin complex to allow membrane-bound p150Glued to activate dynein, since p50 over-expression, which disrupts the dynactin complex, inhibits inward movement even though dynein and p150Glued remain membrane-bound. Long dynein-dependent movements occur via bursts at up to ∼8 µms−1 that are linked by changes in rate or pauses. These peak speeds during rapid inward endosome movement are still seen when cellular dynein levels are 50-fold reduced by RNAi knock-down of dynein heavy chain, while the number of movements is reduced 5-fold. Altogether, these findings identify how dynein helps define the dynamics of early endosomes.
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影响因子：
7.8
作者：
Gross, S P;Welte, M A;Block, S M;Wieschaus, E F
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Wieschaus, E F
DOI：
10.1083/jcb.200908075
发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0706192104
发表时间：
2007-08-21
影响因子：
11.1
作者：
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通讯作者：
Chu, Steven