Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity.
Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity.
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DOI：
10.1016/j.jim.2010.06.017
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发表时间：
2010-08-31
影响因子：
2.2
通讯作者：
Williams, Kenneth C.
中科院分区：
文献类型：
--
作者：
Autissier, Patrick;Soulas, Caroline;Burdo, Tricia H.;Williams, Kenneth C.
在传染病期间监测恒河猴免疫细胞群的变化至关重要。本工作的目的是使用一个12色流式细胞仪面板同时分析恒河猴淋巴细胞，单核细胞和树突状细胞（DC）亚群的表型。用12种抗体的混合物标记来自健康未感染恒河猴的血液。将数据与三个较小的谱系特异性组进行比较，并比较细胞的绝对和相对百分比。我们的12色面板允许识别以下主要亚群：CD 4+和CD 8 + T淋巴细胞、B淋巴细胞、自然杀伤（NK）细胞、自然杀伤T（NKT）细胞、单核细胞亚群和四个非重叠HLA-DR+ Lin-细胞亚群：CD 34+造血干细胞、CD 11 c − CD 123+浆细胞样DC、CD 11 c + CD 16+和CD 11 c −/dim CD 1c+髓样DC。多参数流式细胞术组的开发将允许同时计数成熟淋巴细胞、NK细胞、单核细胞和DC亚群。在一个小组中研究免疫系统的这些主要参与者可以让我们更广泛地了解SIV感染期间的免疫反应，并能够更好地定义这些单个细胞类型在艾滋病发病机制中的作用。
Monitoring changes in rhesus macaque immune cell populations during infectious disease is crucial. The aim of this work was to simultaneously analyze the phenotype of rhesus macaque lymphocyte, monocyte and dendritic cell (DC) subsets using a single 12-color flow cytometry panel. Blood from healthy non-infected rhesus macaques was labeled with a cocktail of 12 antibodies. Data were compared to three smaller lineage specific panels and absolute and relative percentages of cells were compared. Our 12-color panel allows for the identification of the following major subsets: CD4+ and CD8+ T lymphocytes, B lymphocytes, natural killer (NK) cells, natural killer T (NKT) cells, monocyte subsets and four non-overlapping HLA-DR+ Lin- cell subsets: CD34+ hematopoietic stem cells, CD11c− CD123+ plasmacytoid DC, CD11c+ CD16+ and CD11c−/dim CD1c+ myeloid DC. The development of a multiparameter flow cytometry panel will allow for simultaneous enumeration of mature lymphocyte, NK cells, monocyte and DC subsets. Studying these major players of the immune system in one panel may give us a broader view of the immune response during SIV infection and the ability to better define the role of each of these individual cell types in the pathogenesis of AIDS.
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影响因子：
0.7
作者：
Brown KN;Barratt-Boyes SM
通讯作者：
Barratt-Boyes SM
影响因子：
3.7
作者：
Autissier, Patrick;Soulas, Caroline;Williams, Kenneth C.
通讯作者：
Williams, Kenneth C.
影响因子：
2.2
作者：
Della Bella, Silvia;Giannelli, Stefania;Villa, Maria Luisa
通讯作者：
Villa, Maria Luisa
影响因子：
20.3
作者：
MacDonald, KPA;Munster, DJ;Hart, DNJ
通讯作者：
Hart, DNJ
影响因子：
4.4
作者：
Dzionek, A;Fuchs, A;Schmitz, J
通讯作者：
Schmitz, J