Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues.
Chicken RNA-binding protein T-cell internal antigen-1 contributes to stress granule formation in chicken cells and tissues.
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DOI：
10.4142/jvs.2018.19.1.3
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发表时间：
2018-01-31
影响因子：
1.8
通讯作者：
Ding C
中科院分区：
文献类型：
--
作者：
Sun Y;Zhang P;Zheng H;Dong L;Tan L;Song C;Qiu X;Liao Y;Meng C;Yu S;Ding C
T细胞内抗原-1(TIA-1)在调节人类细胞中选择性的前mRNA剪接、mRNA翻译和应激颗粒(SG)的形成中起着重要作用。作为一种进化上保守的对环境胁迫的反应，SGS在各种物种中都有报道。然而，鸡细胞中SG的形成以及鸡TIA-1(CTIA-1)在SG组装中的作用尚不清楚。在本研究中，我们克隆了CTIA-1，并表明它促进了规范的SGS在人和鸡细胞中的组装。带有绿色荧光蛋白(GFP)N端标签(pntGFP-CPRD)或标志标签(PFLAG-CPRD)的CTIA-1蛋白酪蛋白相关结构域(CPRD)的过表达诱导了典型SGS的产生。然而，C-末端的GFP标记的CPRD诱导了明显的大的胞质颗粒，这些颗粒缺乏内源性G3BP1，并且在环己亚胺作用下保持稳定，表明这些不是典型的SGS，并且pntGFP标签影响CPRD的定位。最后，在环境胁迫下，内源性CTIA-1被募集到鸡细胞和组织中的SGS中。综上所述，我们的研究为CTIA-1在鸡细胞和组织中典型的SG形成中发挥作用提供了证据。我们的结果还表明，CPRD对于SG聚集是必要的。
T-cell internal antigen-1 (TIA-1) has roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SGs have been reported in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and chicken cells. Overexpression of the chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal green fluorescent protein (GFP) tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP-tagged cPRD induced notably large cytoplasmic granules that were devoid of endogenous G3BP1 and remained stable when exposed to cycloheximide, indicating that these were not typical SGs, and that the pntGFP tag influences cPRD localization. Finally, endogenous cTIA-1 was recruited to SGs in chicken cells and tissues under environmental stress. Taken together, our study provide evidence that cTIA-1 has a role in canonical SG formation in chicken cells and tissues. Our results also indicate that cPRD is necessary for SG aggregation.
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