Evaluation of a novel non-penetrating electrode for use in DNA vaccination.
Evaluation of a novel non-penetrating electrode for use in DNA vaccination.
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DOI：
10.1371/journal.pone.0019181
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发表时间：
2011-04-29
期刊：
影响因子：
3.7
通讯作者：
Heller R
中科院分区：
文献类型：
--
作者：
Donate A;Coppola D;Cruz Y;Heller R
目前在疫苗开发方面的进展降低了致命性和非致命性感染的发生率，并延长了寿命。然而，需要开发新的技术来对抗新一代的传染病。DNA疫苗已被证明在治疗各种疾病方面具有巨大的潜力。单独使用这项技术不会对疫苗接种产生显著的免疫反应，但与电穿孔传递(EP)相结合，可以增强质粒的表达和免疫力。大多数EP系统虽然有效，但可能是侵入性的和痛苦的，使它们不太适合用于疫苗接种。我们的实验室最近开发了一种名为多电极阵列(MEA)的非侵入性电极，它平放在皮肤表面，不会穿透组织。在这项研究中，我们评估了MEA在以乙肝病毒为感染模型的DNA疫苗接种中的应用。我们使用了豚鼠模型，因为他们的皮肤在厚度和形态上与人类相似。将编码乙肝表面抗原(HBs)的质粒与MEA一起皮内注射到豚鼠皮肤。结果显示，与单独注射相比，使用MEA的质粒组蛋白表达增加。在治疗后48小时内，实验组有大量细胞渗入。与单纯注射组相比，体液反应在持续时间和强度上都显著增加。虽然这种电极还需要进一步的研究，但我们的结果表明，MEA在电介导皮内DNA疫苗中具有潜在的应用前景。
Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use with a wide variety of diseases. Alone, this technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our lab recently developed a non-invasive electrode known as the multi-electrode array (MEA), which lies flat on the surface of the skin without penetrating the tissue. In this study we evaluated the MEA for its use in DNA vaccination using Hepatitis B virus as the infectious model. We utilized the guinea pig model because their skin is similar in thickness and morphology to humans. The plasmid encoding Hepatitis B surface antigen (HBsAg) was delivered intradermally with the MEA to guinea pig skin. The results show increased protein expression resulting from plasmid delivery using the MEA as compared to injection alone. Within 48 hours of treatment, there was an influx of cellular infiltrate in experimental groups. Humoral responses were also increased significantly in both duration and intensity as compared to injection only groups. While this electrode requires further study, our results suggest that the MEA has potential for use in electrically mediated intradermal DNA vaccination.
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